应用液相色谱同时测定土壤中丁醚脲和哒螨灵残留量的新方法研究  

Simultaneous Determination of Diafenthiuron and Pyridaben Residues in Soil by HPLC

作　　者：石卿[1] 郭正元[1] 张舜[1] 刘斐[1] 张宇[1] 

机构地区：[1]湖南农业大学农业环境保护研究所,湖南长沙410128

出　　处：《湖南农业科学》2012年第5期54-56,共3页Hunan Agricultural Sciences

基　　金：江苏克胜集团股份有限公司资助(2010P500)

摘　　要：采用乙腈溶液提取,PSA吸附剂纯化,反相高效液相色谱柱分离,紫外检测器检测,同时测定土壤中丁醚脲和哒螨灵的残留量。以甲醇/水(85/15,V/V)为流动相,选择3个浓度水平进行添加回收率试验。结果表明:丁醚脲的平均添加回收率为96.27%,标准偏差在2.18～11.63之间;哒螨灵的平均添加回收率为91.23%,标准偏差在3.26～6.17之间,丁醚脲和哒螨灵的色谱分离度R>1.5。研究得到了满意效果,该方法操作简便、快速、准确、可靠。Through acetonitrile solution extraction,PSA adsorbent purification,RP-HPLC separation and UV detection,the residues of Diafenthiuron and Pyridaben in soil were determined.Then,the methanol/water（85/15,V/V） was used as mobile phase and three levels were chosen to conduct fortified recovery experiment.The results showed that average recovery rate of Diafenthiuron was 96.27% and its standard deviation was 2.18~11.63;average recovery rate of Pyridaben was 91.23% and its standard deviation was 3.26~6.17;the chromatographic resolution degree（R） between Diafenthiuron and Pyridaben was more than 1.5.

关 键 词：丁醚脲 哒螨灵 反相高效液相色谱 土壤残留检测 

分 类 号：S482.3[农业科学—农药学]