金欣口服液含药血清对呼吸道合胞病毒感染RAW264.7细胞Toll样受体3表达的调控作用  

Regulation effect of Jinxin Oral Liquid Drug Serum on the expression of Toll like receptor 3 in RAW264.7 cells infected with RSV

作　　者：李佳曦[1] 汪受传[1] 徐建亚[1] 戴启刚[1] 徐珊[1] 孙寒丹[1] 彭璐璐[2] 陈争光[1] 

机构地区：[1]南京中医药大学中医儿科研究所,南京210029 [2]南京师范大学生命科学学院,南京210046

出　　处：《中华中医药杂志》2012年第6期1646-1649,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81072840);全国高等学校博士学科点专项科研基金(No.20103237110003);江苏省普通高校研究生科研创新计划(No.CXZZ11-0773)~~

摘　　要：目的:通过研究金欣口服液含药血清对呼吸道合胞病毒(RSV)活化诱导的Toll样受体3(TLR3)的干预作用,探讨其治疗RSV肺炎可能的免疫学机制。方法:RSV感染体外培养的RAW264.7细胞,采用金欣口服液含药血清进行干预,并设利巴韦林阳性对照,24h后收集细胞,Real time RT-PCR法测定TLR3 mRNA的表达变化;激光共聚焦显微镜观察免疫荧光细胞化学染色法处理的各组细胞TLR3表达水平;ELISA法检测各组细胞培养上清中白细胞介素-6(IL-6)含量,在核酸及蛋白水平探讨金欣口服液含药血清抗RSV感染的作用机制。结果:RSV感染RAW264.7细胞后24h,细胞中TLR3 mRNA和蛋白表达水平以及细胞培养上清中IL-6表达明显升高(P<0.01),而金欣组TLR3及IL-6表达均显著低于RSV感染组(P<0.01,P<0.05),且效果优于利巴韦林组(P<0.01,P<0.05)。结论:金欣口服液含药血清能下调RSV活化诱导的TLR3及其下游炎症因子IL-6的高表达,推测其为金欣口服液抗RSV感染的机制之一。Objective： To investigate the regulation effect of Jinxin oral liquid drug serum on the expression of Toll like receptor 3 （TLR3） in RSV infected RAW264.7 cells. Methods： RAW264.7 cells were infected with RSV, and treated with the drug serum of Jinxin oral liquid, and set ribaviran positive control. 24 hours later, detected the level of TLR3 mRNA and protein with Real time RT-PCR and immunofluorescence （IF） assay; collected the supernatant and measured the level of Interleukin 6 （IL-6）. Results： The expression of TLR3 mRNA and protein and IL-6 was up-regulated significantly compared with the control group（P〈0.01）, but they were typically down-regulated in Jinxin oral liquid groups compared with RSV infected groups（P〈0.01, P〈0.05）, and better than ribaviran group （P〈0.01, P〈0.05）. Conclusion： TLR3 signaling pathway maybe the target of Jinxin oral liauid in anti-RSV infection.

关 键 词：金欣口服液 含药血清 呼吸道合胞病毒 TOLL样受体3 白细胞介素-6 小鼠肺巨噬细胞 

分 类 号：R285.5[医药卫生—中药学]