记忆提取对小鼠树突棘的形态学影响  

Effect of Memory Recall on the Morphology of Dendritic Spines in Mice

作　　者：张晓光[1] 薛张纲[1] 

机构地区：[1]复旦大学附属中山医院麻醉科,上海200032

出　　处：《中国临床医学》2012年第2期91-93,共3页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金资助(编号:81100796)

摘　　要：目的:观察Morris水迷宫位置回想测试对树突棘的形态学影响。方法:12只6～7个月龄雄性绿色荧光蛋白转基因小鼠在接受Morris水迷宫定位航行训练至空间记忆形成后,将其随机分为位置回想测试组(n=6)和对照组(n=6)。观察2组小鼠树突棘分布密度的改变。结果:位置回想测试组小鼠树突棘密度为1.69(1.47～2.00)个/μm(18个细胞,65个树突节段,3 034个树突棘);对照组小鼠树突棘密度为1.33(1.04～1.55)个/μm(18个细胞,66个树突节段,2 723个树突棘)。位置回想测试组小鼠海马CA1区锥体细胞树突棘的密度显著大于对照组小鼠。结论:记忆提取可以增加小鼠海马CA1区锥体细胞树突棘的密度,提示记忆提取过程可能伴有兴奋性突触的数量增加。Objective：To investigate the effect of memory recall on the morphology of dendritic spines in green fluorescent protein（GFP） transgenic mice.Methods：Twelve male GFP transgenic mice received spacial navigation training in Morris water maze until they met the memory formation criteria.Then the mice were randomly allocated to either the place recall test group（n=6） or the control group（n=6）.Morphology of dendritic spines was observed through laser confocal microscopy.Densities of dendritic spines were compared between the two groups.Results： The density of dendritic spines in the test group was 1.69（1.47-2.00） spines/μm（18 cells,65 dendritic segments,3 034 spines） and the density of dendritic spines in the control group was 1.33（1.04-1.55）spines/μm（18 cells,66 dendritic segments,2 723 spines）.The density of dentritic spines in the test group was greater than that in the control group（P〈0.05）.Conclusions： Memory recall process can increase the density of dendritic spines in pyramidal cells in the hippocampus CA1 region of mice,which indicates that memory recall process may be accompanied by formation of new excitatory synapses.

关 键 词：MORRIS水迷宫 树突棘 记忆回想 

分 类 号：R614.21[医药卫生—麻醉学]