慢性阻塞性肺病外周血单个核细胞表面TLR2、TLR4的表达与血清TNF-α、IL-6、IL-8相关性的研究  

Expression of TLR2 and TLR4 on the Surface of Monocytes were Associated with Serum Levels of TNF-α,IL-6 and IL-8 in Patients with Chronic Obstructive Pulmonary Disease

作　　者：龚燕[1] 施小梅[1] 顾洁[1] 刘江霞[2] 金美玲[3] 蔡映云[4] 刘宏炜 

机构地区：[1]复旦大学附属华山医院老年科,上海200040 [2]复旦大学上海医学院医学分子病毒实验室,上海200032 [3]复旦大学附属中山医院呼吸科,上海200032 [4]复旦大学附属中山医院老年科,上海200032 [5]上海市奉贤区中心医院呼吸科,上海201400

出　　处：《中国临床医学》2012年第2期108-111,共4页Chinese Journal of Clinical Medicine

基　　金：上海市卫生局资助项目(编号:2009010)

摘　　要：目的:探讨慢性阻塞性肺病(chronic obstructive pulmonary disease,COPD)患者外周血单个核细胞表面Toll样受体(Toll-like receptors)TLR2和TLR4的表达情况及其与血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)的关系。方法:本研究涉及的3组研究对象分别为:吸烟COPD组(n=68)、吸烟非COPD组(n=42)和不吸烟非COPD组(n=43)。将68例吸烟COPD患者进一步分为急性发作期亚组(n=32)和稳定期亚组(n=36)。采用实时荧光定量PCR法检测外周血单个核细胞表面TLR2、TLR4的表达水平,同时采用酶联免疫吸附试验(ELISA)试剂盒测定血清细胞因子的浓度。结果:吸烟COPD组外周血单个核细胞表面TLR2、TLR4的表达水平及血清TNF-α、IL-6、IL-8的浓度均显著高于吸烟非COPD组(TLR2:1.87±2.04比0.47±0.24;TLR4:4.08±3.84比0.41±0.18;TNF-α:68.20±56.26比26.20±5.43;IL-6:201.62±174.69比72.99±8.03;IL-8:235.42±158.22比77.50±8.38)。吸烟非COPD组外周血单个核细胞上TLR2、TLR4的表达水平均显著低于不吸烟非COPD组(TLR2:0.47±0.24比2.96±2.71;TLR4:0.41±0.18比2.67±1.74),而吸烟非COPD组血清TNF-α、IL-6、IL-8的浓度均显著高于不吸烟非COPD组(TNF-α:26.60±5.43比8.93±6.41;IL-6:72.99±8.03比25.61±8.63;IL-8:77.50±8.38比47.10±11.11)。COPD急性发作期单个核细胞表面TLR2、TLR4的表达水平及血清TNF-α、IL-6、IL-8的浓度均显著高于COPD稳定期(TLR2:3.56±1.80比0.368±0.222;TLR4:6.97±3.76比1.51±0.943;TNF-α:106.17±61.94比90.01±19.66;IL-6:327.18±183.56比105.36±13.45;IL-8:381.74±108.51比34.44±10.45)。吸烟COPD组外周血单个核细胞表面TLR2、TLR4的表达水平与血清TNF-α、IL-6、IL-8的浓度成正相关。结论:COPD患者尤其是COPD急性发作期患者外周血单个核细胞表面TLR2、TLR4的表达水平及血清TNF-α、IL-6、IL-8的浓度均显著升高;并且COPD患者外周血单个核细胞表面TLR2、TLR4的表达水平与血清TNF-α、IL-6、IL-8的浓度成正相�Objective：To investigate the expression of Toll-like receptor（TLR）2 and TLR4 on the surface of peripheral blood mononuclear cells,as well as the serum levels of tumor necrosis factor alpha（TNF alpha）,interleukin 6（IL-6）,interleukin 8（IL-8） in patients with chronic obstructive pulmonary disease（COPD）.Methods： There were three groups in this study： smoking COPD group,smoking healthy group and non-smoking healthy group.The COPD group was divided into acute period group and stable period group.Real-time fluorescence quantitative PCR assay was used to detect the expression of TLR2 and TLR4,while enzyme-linked immunosorbent assay（ELISA） kits were used to detect serum cytokine concentrations.Results： TLR2 and TLR4 expression levels,as well as serum concentrations of TNF-α,IL-6 and IL-8 were significantly higher in smoking COPD group than in smoking healthy group（P〈0.01）.TLR2 and TLR4 expression levels were significantly lower in smoking healthy group than in non-smoking healthy group,while TNF-α,IL-6,IL-8 concentrations in smoking healthy group were significantly higher compared with non-smoking healthy group（P〈0.01）.The expression levels of TLR2 and TLR4,as well as the serum concentrations of TNF-α,IL-6 and IL-8 were significantly higher in acute period COPD group than in stable period COPD group（P〈0.01）.TLR2 and TLR4 expression levels were positively correlated with serum concentrations of TNF-α,IL-6 and IL-8 in COPD patients.Conclusions： TLR2,TLR4 expression levels and serum levels of TNF-α,IL-6,IL-8 were significantly increased in COPD patients.It is more significant in acute period COPD.TLR2 and TLR4 expression levels were positively related to serum levels of TNF-α,IL-6 and IL-8.COPD’s occurrence and development are correlated with natural immunity.

关 键 词：慢性阻塞性肺病 TOLL样受体 肿瘤坏死因子-α 白细胞介素-6 白细胞介素-8 

分 类 号：R563[医药卫生—呼吸系统]