3株超耐硒细菌的分离 筛选与鉴定  

Isolation,Screening,Identification of Three Strains With High-Se Tolerance

作　　者：彭祚全 樊俊[2] 向德恩[2] 施河丽[2] 向必坤[2] 左梅[2] 

机构地区：[1]恩施市疾病预防控制中心 [2]湖北省烟草公司恩施州公司,湖北恩施445000

出　　处：《微量元素与健康研究》2012年第3期4-6,共3页Studies of Trace Elements and Health

摘　　要：目的:在高硒区自然环境中寻找超耐硒并能还原硒的微生物。方法:通过对湖北省恩施市新塘乡渔塘坝硒矿床带采集的10种检样分别进行富集、分离、筛选、纯化培养出具有超耐硒能力的菌株3株,经鉴定分别为鲍氏不动杆菌、地衣芽胞杆菌和枯草芽胞杆菌。初步研究了这3株菌的培养特性、形态特征和生化学与分子生物学性状。结果:地衣芽胞杆菌和枯草芽胞杆菌在含硒量为33000μg/ml的营养琼脂培养基中能正常生长并能还原无机硒为红色单质硒,在含硒量为46500μg/ml时仍能缓慢生长。结论:超耐硒微生物的发现,在治理环境硒污染、富硒农产品开发等方面具有重要意义。Objective： To screen out the strains that can tolerant high dose of selenium in the high - Se environment of Yutangba. Methods： Three strains were screened out by isolation and purification culture. The strains were identified as Acinetobacter baumannii, Bacillus licheniformis and Bacillus subtilis. Result ： Characteristics of the strains were studied and two of them had the ability to tolerant and reduce selenium of 33000 μg/ml. They grew slowly in the selenium of 46500 μg/ml. Conclusions： The finding of the strains played an important role in the control of high- Se environment pollution and the supplement of selenium in Agriculture products.

关 键 词：高硒区 超耐硒 菌株 

分 类 号：O613.52[理学—无机化学]