Improved biocompatibility of poly(vinylpyrrolidone)-graft-poly(2-dimethylaminoethyl methacrylate)/DNA complexes by coating with bovine serum albumin  

作　　者：Biao Zhang Lian Dong Deng Jin Feng Xing Jun Yang An Jie Dong 

机构地区：[1]Department of Polymer Science and Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China [2]The Key Laboratory of Bioactive Materials,Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China

出　　处：《Chinese Chemical Letters》2012年第5期627-630,共4页中国化学快报（英文版）

基　　金：the support of National Natural Science Foundation of China(No31100722);Specialized Research Fund for the Doctoral Program of Higher Education(No20090032120013)

摘　　要：Bovine serum albumin（BSA） was utilized to assemble with the binary complexes of poly（vinylpyrrolidone）-graft-poly（2- dimethylaminoethyl methacrylate）（PVP-g-PDMAEMA）/DNA formed by layer-by-layer electrostatic interactions to screen the residual surface positive charges of complexes.The coating of BSA was able to decrease the zeta potential of binary complexes nearly to electroneutrality without interfering with DNA condensation ability.The ternary complexes of BSA/PVP-g-PDMAEMA/ DNA demonstrated lower cytotoxicity compared with the binary complexes and also maintained high gene transfection efficiency in HepG2 cells.Bovine serum albumin（BSA） was utilized to assemble with the binary complexes of poly（vinylpyrrolidone）-graft-poly（2- dimethylaminoethyl methacrylate）（PVP-g-PDMAEMA）/DNA formed by layer-by-layer electrostatic interactions to screen the residual surface positive charges of complexes.The coating of BSA was able to decrease the zeta potential of binary complexes nearly to electroneutrality without interfering with DNA condensation ability.The ternary complexes of BSA/PVP-g-PDMAEMA/ DNA demonstrated lower cytotoxicity compared with the binary complexes and also maintained high gene transfection efficiency in HepG2 cells.

关 键 词：COPOLYMER Electrostatic interaction Bovine serum albumin Gene delivery 

分 类 号：TQ655[化学工程—精细化工] O632.52[理学—高分子化学]