The Analysis for the Single Nucleotide Polymorphism of TGF-β1 and Its Association with Reproduction in Different Sheep Breeds  

作　　者：LixiaGao HongbinLi LixinDu XuemeiSong ZhenzuLi ShangangLi CaihongWei 

机构地区：[1]NationalCenterforMolecularGeneticsandBreedingofAnimal,InstituteofAnimalScience,ChineseAcademyofAgriculturalSciences,Beijing,100193,China [2]CollegeofLifeSciences,ShandongNormalUniversity,Jinan,Shandong,250014,China

出　　处：《Journal of Animal Science and Biotechnology》2010年第2期70-74,共5页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30270949);"863"Plan(No.2006AA10Z199)

摘　　要：A single nucleotide polymorphism （SNP） of 805 bp region in the intron 6 of transfor- ming growth factor β1 （TGF-fll） gene was identified by polymerase chain reaction-single-strand composi- tion polymorphism （PCR-SSCP） in 196 sheep among Small-tailed Han sheep, Tong sheep, Tan sheep and Oula sheep. Comparative sequence analysis of cloned products revealed an AGAC deletion at 294 bases up-stream of exon 7 of the TGF-βI gene （ site 14201 in gi76871756）. Statistical results of the genotype and allele frequencies in different breeds showed that gen- otype AB was dominant in the Small-tailed Han sheep. Genotype BB, however, was in majority in low- fecundity sheep. The results of a Chi-square test indi- cated that all the populations were in Hardy-Weinberg equilibrium.A single nucleotide polymorphism （SNP） of 805 bp region in the intron 6 of transfor- ming growth factor β1 （TGF-fll） gene was identified by polymerase chain reaction-single-strand composi- tion polymorphism （PCR-SSCP） in 196 sheep among Small-tailed Han sheep, Tong sheep, Tan sheep and Oula sheep. Comparative sequence analysis of cloned products revealed an AGAC deletion at 294 bases up-stream of exon 7 of the TGF-βI gene （ site 14201 in gi76871756）. Statistical results of the genotype and allele frequencies in different breeds showed that gen- otype AB was dominant in the Small-tailed Han sheep. Genotype BB, however, was in majority in low- fecundity sheep. The results of a Chi-square test indi- cated that all the populations were in Hardy-Weinberg equilibrium.

关 键 词：PCR-SSCP SHEEP SNP TGF-Β1 

分 类 号：Q987[生物学—遗传学] S826.2[生物学—人类学]