降脂饮颗粒中何首乌有效成分的测定  

Determination of effective content of polygonum multiflorum in lipid-lowering drink particles

作　　者：柳青 任德玉 曹学东 刘丽凤[1] 

机构地区：[1]武警山西总队医院药剂科,太原030006 [2]武警山西总队后勤部卫生处,太原030012 [3]山西省中西医结合医院,太原030006

出　　处：《武警医学》2012年第5期389-391,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的建立降脂饮颗粒中何首乌有效成分的测定方法。方法采用HPLC法,以十八烷基硅烷键合硅胶为填充剂;以乙腈-水(25∶75)为流动相;检测波长为320 nm;柱温25℃;流速为1.0 ml/min,测定何首乌中主要成分2,3,5,4'-四羟基二苯乙烯-2-0-β-D-葡萄糖苷(简称:二苯乙烯苷)的含量。结果二苯乙烯苷在0.050～0.625μg范围内呈良好的线性关系,样品分离度好,与阴性无干扰且样品稳定性好。结论本方法简便、准确、重现性好,适用于降脂饮颗粒中二苯乙烯苷的测定。Objective To establish a method of determining the effective component of Polygonum muhiflorum in lipid - low- ering drink particles. Methods HPLC method was used with oetadeeyl silane bonded silica as a filler and with acetonitrile water （25： 75） as the mobile phase. The detection wavelength was set at 320 nm; the column temperature was 25℃ at a flow rate of 1.0 ml/min. Results 2,3,5,4＇ - tetrahydroxy - stilbene - 2 - 0 - β - D - glucoside in the 0.05 - 0. 625μg range showed a good linear relationship. The sample separation was good, with no interference and sample stability in the negative was good. Conclusions The method is user- friendly and accurate with a good reproducibility. It can be used to determine the content of 2,3,5,4＇ -tetrahydroxy diphenyl ethylene - 2 - 0 -β - D - glucoside.

关 键 词：何首乌 2 3 5 4'-四羟基二苯乙烯-2-0-β-D-葡萄糖苷 高效液相色谱法 有效成分 

分 类 号：R282.71[医药卫生—中药学]