不同阶段兔主动脉粥样硬化斑块的一氧化氮合酶活性及其mRNA表达  

Activity and mRNA Expression of Nitric Oxide Synthase in Different Phases of Atherosclerotic Plaques

作　　者：李亚俊[1] 宋剑南[1] 牛晓红[1] 王宇辉[1] 王少君[1] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700

出　　处：《中国动脉硬化杂志》2000年第1期13-16,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金! (项目编号 39370 82 )

摘　　要：为探讨一氧化氮合酶与动脉粥样硬化斑块形成过程的相互关系 ,采用酶组织化学染色法和原位杂交法观察实验第 8周、12周和 16周三个阶段的兔主动脉粥样硬化斑块一氧化氮合酶活性及其 16周时的mRNA表达。结果显示 ,实验 8周时 ,主动脉斑块内一氧化氮合酶活性呈现弱阳性、阳性和强阳性三种不同的表现形式 ,并相应有不同的细胞分布方式 ;实验 12周时 ,一氧化氮合酶活性在斑块中广泛分布 ,且于中、内膜交界处更为明显 ;实验16周时 ,主动脉脂质斑块中一氧化氮合酶活性及其mRNA表达明显下降 ,此阶段的大面积mRNA表达阴性主要与脂质坏死中心的大量形成有关。提示动脉粥样硬化血管壁的一氧化氮合酶活性和基因表达是一个不断变化的动态过程 ,且因细胞类型和分布部位而异。Aim [WT5”BZ]To study the changes in activity and mRNA expression of nitric oxide synthase (NOS) in different phases of atherosclerotic lesion.[WT5”HZ] Methods [WT5”BZ]24 male New Zeland white rabbits were divided into normal control group(6 rabbits) and hypercholesterol group(18 rabbits). At the time of the 8th, 12th and 16th week, 6 rabbits of hypercholesterol group were sacrificed. Using NADPH-diaphorase histochemistry, we studied the activity of NOS. The NOS mRNA expression in aortas in the 16th week was also studied with in situ hybridization.[WT5”HZ] Results [WT5”BZ]In the 8th week, in cholesterol group, there were three different aspects of NADPH-d stains in plaques, which were doubtful positive, positive and strong positive. In the 12th week, there were extensive NADPH-d moderate positive stains in plaques, particularly at the border of intima and media. In the 16th week, the activity and mRNA expression of NOS in both intima and media decreased accompanying with the forming of necrosis core.[WT5”HZ] Conclusion [WT5”BZ]Changes in activity and expression of NOS in atherosclerotic lesion are dynamic with different locations of cells.

关 键 词：动脉粥样硬化 主动脉 一氧化氮合酶 基因表达 

分 类 号：R543.5[医药卫生—心血管疾病]