三七总皂甙对人高脂血清诱发的胎儿血管平滑肌细胞增殖的抑制作用  

Inhibitory Effect of Panax Notoginseng Saponins on Proliferation of Cultured Vascular Smooth Muscle Cells Stimulated by Serum of Hyperlipidemia

作　　者：周晓霞[1] 苏佩清[1] 杨鹤梅[1] 周晓慧[1] 

机构地区：[1]承德医学院生物化学教研室,河北省承德市067000

出　　处：《中国动脉硬化杂志》2000年第1期43-45,共3页Chinese Journal of Arteriosclerosis

基　　金：河北省科委资助!(课题号 962 1 61 0 7D)

摘　　要：为观察三七总皂甙对高脂血清刺激的人胎儿血管平滑肌细胞增殖的抑制作用。利用体外细胞培养技术 ,用人高脂血清造成血管平滑肌细胞增殖的模型并通过MTT测定、细胞计数及透射电镜下的细胞超微结构的观察等方法 ,观察了三七总皂甙对人胎儿血管平滑肌细胞的保护作用。结果发现 ,人高脂血清可明显刺激人胎儿血管平滑肌细胞的增殖并引起其表型的改变。而三七总皂甙可呈浓度依赖性地抑制血管平滑肌细胞的增殖并可显著抑制高脂血清对血管平滑肌细胞的促增殖作用。此结果提示 ,三七总皂甙的抗动脉粥样硬化作用可能部分是通过抑制血管平滑肌细胞异常增殖来实现的。Aim [WT5”BZ]To investigate the protective effect of Panax Notoginseng Saponins (PNS) on the cultured aortic smooth muscle cells (SMC). [WT5”HZ]Methods [WT5”BZ]In this study, cell culture technique was used in vitro. The protective effect of PNS on the proliferation of SMC was observed by measuring MTT metabolism and cell numbers and observing cell ultrastructure under TEM. [WT5”HZ]Results[WT5”BZ] The serum hyperlipidemia caused the increases in cell numbers and MTT metabolism of SMC. Under TEM, there are numerous rough endoplasmic reticulum and mitochondria and lipids in the cytoplasm of the cells cultured by serumof hyperlipidemia. The PNS can inhibit the proliferation of SMC dose dependently and can inhibit the proliferation of SMC stimulated by serumof hyperlipidemia significantly. [WT5”HZ]Conclusions[WT5”BZ] The PNS may prevent atherosclerosis and inhibit progression of the atheroslerotic lesions by interfering with the proliferation of arterial SMC.

关 键 词：血管平滑肌 三七总皂甙 药理学 细胞培养 

分 类 号：R285.5[医药卫生—中药学]