结核杆菌特异性单克隆抗体研究  

Study on specific anti tubercular bacilli monoclonal antibodies

作　　者：李登旭[1] 王春俭[2] 彭义利[1] 王征帆[3] 朱宝玉[2] 姚党生[2] 马士文 

机构地区：[1]河南省结核病防治研究所,河南郑州450003 [2]河南省卫生防疫站,河南郑州450003 [3]河南省肿瘤医院,河南郑州450003 [4]河南省卫生厅,河南郑州450003

出　　处：《河南医学研究》2000年第1期15-16,共2页Henan Medical Research

基　　金：河南省医学科学院科研基金

摘　　要：目的 :制备结核菌特异性单克隆抗体 ;检测患者胸 (腹 )水、脑脊液中结核菌特异性抗原。方法 :制备特异性单克隆抗体 ,制备兔抗结核菌高效价多克隆抗体血清 ;DOT -ELISA法检测患者胸、腹水或脑脊液中结核菌特异性抗原。结果 :检测结核性胸 (腹 )水、结核性脑膜炎和脑结核球患者的脑脊液、恶性胸水、脑囊虫患者的脑脊液 ,结核菌抗原检查阳性率分别为 75 %、10 0 %、5 8%、0 %和 0 %。结论 :为结核性胸 (腹 )膜炎 ,结核性脑膜炎、脑结核球的诊断和鉴别诊断提供了快速、简便。Objective: To study the preparation of anti tubercular bacilli monoclonal antibodies (McAb),and to detect the specific tubercular bacilli antigen in cerebrospinal fluid (CSF) and pleural effusion or ascited.Methods: Preparing specific tubercular bacilli McAb.Preparing rabbit serum of high efficiency multiclonal antibodies of antitubercular bacilli.Detecting specific tubercular bacilli antigen in CSF or pleural effusion or ascites of tuberculosis cases,using DOT ELISA method.Results: The tubercular bacilli antigen positive rate in tubercular pleural effusion (or ascites),CSF of tubercular meningitis,CSF of cerebral tuberculoma,malignant effusion and CSF of cases with cerebral systicersis is 75%,100%,58%,0%,0%,respetively.Conclusion: The detection of specific tubercular bacilli antigen provides rapid,simple and accurate detection method for the diagnosis and differentiation of tubercular pleural effusion (or ascites),meningitis and cerebral tuberculoma.

关 键 词：结核菌 单克隆抗体 诊断 

分 类 号：R520.4[医药卫生—内科学]