18β-甘草次酸对HO-8910PM细胞Fas/FasL表达的影响  

Effects of 18β-glycyrrhetinic Acid on the Expression of Fas and FasL in HO-8910PM cells

作　　者：陈杰[1] 庞江琳[2] 覃燕梅[1] 梁念慈[1] 

机构地区：[1]广东医学院,广东湛江524023 [2]广东医学院附属医院,广东湛江524023

出　　处：《时珍国医国药》2012年第5期1056-1058,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.39870900);广东省重点学科基金资助(No.Gx0301)

摘　　要：目的研究18β-甘草次酸(18β-glycyrrhetinic acid,18β-GA)对人高转移卵巢癌HO-8910PM细胞中Fas,FasL表达的影响,探讨Fas,FasL对卵巢癌细胞凋亡的作用机制。方法不同浓度药物作用HO-8910PM细胞,MTT法检测细胞的生长增殖,流式细胞仪检测细胞周期,FITC法检测Fas,FasL的蛋白表达情况。结果 18β-GA对高转移卵巢癌细胞HO-8910PM的生长和增殖具有显著的抑制作用;药物使8910PM细胞周期阻滞于G1期,凋亡增加。FITC检测发现Fas,FasL蛋白表达上调(P<0.05)。结论 18β-GA通过上调HO-8910PM中Fas,FasL蛋白表达,促使细胞凋亡,抑制细胞增殖,提示18β-GA可能成为治疗卵巢癌的潜在药物。Objective To investigate the effect and mechanism of 18β-glycyrrhetinic acid（18β-GA）on the proliferation and apoptosis of HO-8910PM cells.Methods HO-8910PM cells were treated by different final concentrations of 18β-GA for 12,24 h.The effect on the proliferation of HO-8910PM cells was assessed by MTT assay.The cell cycle and the protein expression of Fas,FasL were examined by flow cytometry（FCM）.Results 18β-GA inhibited the proliferation of HO-8910PM cell in concentration-and-time-dependent manner（P〈0.05）.18β-GA increased the ratio of cell at the G0/G1 phase,induced the apoptosis of HO-8910PM cell and increased the expression of Fas and FasL（P〈0.05）.Conclusion 18β-GA inhibits proliferation by G0/G1 phase arrest and induces apoptosis via up-regulating the expression of Fas and FasL in HO-8910PM cells.

关 键 词：18β-GA 凋亡 FAS FASL 卵巢癌 

分 类 号：R962[医药卫生—药理学]