米氏凯伦藻甲醇氯仿提取物的细胞毒性分析  

Cytotoxicity of Karenia mikimotoi extracts

作　　者：刘婷婷[1] 史战鹏[1] 李宏业[1] 刘洁生[1] 杨维东[1] 

机构地区：[1]暨南大学生物工程学系水体富营养化与赤潮防治广东省高校重点实验室,广州510632

出　　处：《卫生研究》2012年第3期405-409,共5页Journal of Hygiene Research

基　　金：国家自然科学基金-广东省联合基金重点资助项目(No.U0733006);国家自然科学基金(No.30970502);中央高校基本科研业务费专项资金

摘　　要：目的研究米氏凯伦藻提取物对3株肿瘤细胞的增殖抑制作用,分析米氏凯伦藻对人类健康的可能威胁。方法以体外培养的人宫颈癌细胞HeLa、人肝癌细胞HepG2和人肺癌细胞A549为研究对象,采用MTT比色法检测米氏凯伦藻中提取物对3株细胞增殖的影响;观察毒素在不同时间(12、24、36和48h)下对肿瘤细胞增殖的抑制情况;采用免疫荧光染色法分析比较3株细胞膜上GM1的相对含量。结果米氏凯伦藻提取物对3种肿瘤细胞的增殖均具有显著的抑制作用。与肝癌细胞HepG2相比,宫颈癌细胞HeLa和肺癌细胞A549对其更为敏感(P<0.01)。肝癌细胞HepG2、宫颈癌细胞HeLa和肺癌细胞A549细胞膜上GM1的相对含量差异不大(P>0.05)。结论米氏凯伦藻提取物具有明显的细胞毒性,对人类健康存在潜在威胁。米氏凯伦藻提取物在细胞膜上作用靶点比较复杂。Objective To investigate the potential threat of K. mikimotoi on human health by analyzing the negative effects of K. mikimotoi extract on three cancer cells, HepG2, HeLa and A549. Methods Inhibitory effect of K. mikimotoi extract on the proliferation of three cancer cells was observed by MTT assay and the relative amount of GM1 in cancer cell membrane was detected by immunofluorescence method. Results The extracts of K. mikirnotoi significantly inhibited the proliferation of HeLa, HepG2 and A549 cells in a dose-and time-dependent manner. HeLa and A549 cells were more sensitive than HepG2 cell to the toxicity of K. mikimotoi extracts. However, there was no evident correlation between the proliferative inhibition and the amount of GM1 in cancer cell membrane. Conclusion There was a significant cytotoxicity of K. mikimotoi extracts to mammalian cells, which suggested that their potential threats might be existed to human health. However, the cytotoxic targets of K. mikimotoi extract on cell membrane were complex.

关 键 词：米氏凯伦藻 细胞毒性 增殖 

分 类 号：R994.6[医药卫生—毒理学] R730.1[医药卫生—药学]