正交试验优选蜂病康合剂壳聚糖纯化工艺  

Optimization of Purification Process for Chitosan from Fengbingkang Mixture by Orthogonal Test

作　　者：贾永艳[1] 祝侠丽[1] 陈波[1] 田效志[1] 苗明三[1] 樊静静[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第11期32-34,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省重点科技攻关计划项目(072102130006)

摘　　要：目的:探讨壳聚糖澄清剂用于蜂病康提取液的纯化工艺。方法:以纯化后药液中黄芩苷含量为指标,采用正交试验优选絮凝澄清条件,并与传统醇沉工艺比较。结果:正交优选工艺优于醇沉工艺,最佳絮凝澄清条件为水提液浓缩到1∶8,壳聚糖用量15%,温度50℃。结论:壳聚糖澄清剂可用于蜂病康合剂的精制。Objective：To investigate purification technology of Fengbingkang mixture with chitosan as clarification agent.Method： With the content of baicalin from purified liquid as index,flocculation clarification process was optimized by orthogonal design,and compared with traditional ethanol precipitation process.Result： These test result showed that optimized technology was better than ethanol precipitation technology.Optimum flocculation clarification process conditions were：water extraction liquid concentrated to 1∶8,the amount of chitosan 15%,temperature 50 ℃.Conclusion： Chitosan clarifying agent could be used for purifying Fengbingkang mixture.

关 键 词：壳聚糖 正交实验 蜂病康合剂 纯化工艺 

分 类 号：R283.6[医药卫生—中药学]