一测多评法与外标法测定新清宁片中大黄蒽醌类成分含量  

Determination of Rhubarb Anthraquinones in Xinqingning Tablets by QAMS and External Standard Method

作　　者：张锴镔[1,2] 冯伟红[1,2] 王智民[1,2] 张启伟[1,2] 李霞[3] 杨菲[1,2,4] 李东影[1,2,4] 吉丽娜[1,2,4] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中药质量控制技术国家工程实验室,北京100700 [3]中国中医科学院实验药厂,北京100700 [4]天津中医药大学,天津300193

出　　处：《中国实验方剂学杂志》2012年第11期61-65,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2007年中医药行业科技专业项目(200707009);国家"重大新药创制"科技重大专项(2009ZX09301-005;2009ZX09308-003)

摘　　要：目的:采用"一测多评"法与外标法测定新清宁片中4种大黄蒽醌类成分的含量,并对测定结果进行比较。方法:采用RP-HPLC,Accurasil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.4%磷酸水溶液(85∶15),流速1 mL.min-1,柱温30℃,检测波长254 nm。结果:20批新清宁片中4种蒽醌类成分QAMS计算值与ESM实测值无显著性差异,RSD<5%。结论:在一定色谱条件下,QAMS确定的相对校正因子可作为常数用于含大黄制剂的质量评价。Objective： To determine the contents of four kinds of anthraquinone components in Xinqingning tablet by quantitative analysis of multi-component with a single-marker （QAMS） and external standard method （ESM） respectively, and compare results by this two methods. Method： RP-HPLC determination was performed at 30℃ with Accurasil C18 column （4.6 mm × 250 mm, 5μm） , mobile phase of methanol-0.4% phosphoric acid （85：15） , detection wavelength at 254 rim, and flow rate of 1.0 mL·min-1. Result： There was no significant difference between calculated value of QAMS and measured value of ESM for four kinds of anthraquinone components from Xinqingning tablet, RSD 〈 5%. Conclusion： Under certain chromatographic conditions, relative correlation factor could be used as a constant for quality evaluation of Rheum palmatum preparation which was determined by QAMS.

关 键 词：“一测多评”法 新清宁片 蒽醌 相对校正因子 相对保留值 外标法 

分 类 号：R283.6[医药卫生—中药学]