整体柱HPLC同时测定白葛胶囊中4种有效成分  

Simultaneous Determination of Four Active Components in Baige Capsules by HPLC and Monolithic Column

作　　者：王园姬[1] 周明瑶[1] 韩省力[1] 卢闻[1] 

机构地区：[1]西安交通大学医学院,西安710061

出　　处：《中国实验方剂学杂志》2012年第11期82-85,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省"13115"科技创新工程重大科技专项(2010ZDKG-105)

摘　　要：目的:建立一种同时快速测定白葛胶囊中葛根素、大豆苷元、欧前胡素及异欧前胡素含量的方法。方法:采用C18整体柱(4.6 mm×50 mm,2μm),甲醇(A)-0.1%冰醋酸(B)为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长254 nm,进样体积5μL,柱温30℃。结果:在建立的色谱条件下,4种有效成分可获得良好的分离,且各自在一定的浓度范围内线性关系良好(r>0.9995),方法回收率在95%～105%。结论:所建立的方法简便、快速、可靠,可用于白葛胶囊中多种成分的快速检测。Objective： a method of simultaneous and fast determination the contents of puerarin, daidzein, imperatorin and isoimperatorin in Baige capsules was established. Method： the monolithic C18column was used to perform the separation, and the mobile phase was methanol （A） -0. 1% glacial acetic acid （B） with gradient elution, the flow rate was 1.0 mL·min^-1, the detection wavelength was at 254 nm and the injection volume was 5μL, the column temperature was 30 ℃. Result： these four components could be well separated under the established chromatographic condition, the linear correlation of each component was fine in certain concentration （r 〉0. 999 5）, the recovery was between 95%-105%. Conclusion： the established method was simple, rapid and reliable and could be successfully applied in the active component detection in Baige capsules.

关 键 词：葛根素 欧前胡素 大豆苷元 异欧前胡素 整体柱 

分 类 号：R284.1[医药卫生—中药学]