右美托咪啶预先给药和后处理对大鼠肾缺血再灌注损伤的影响  

Effects of dexmedetomidine pretreatment and postconditioning on renal ischemia-reperfusion injury in rats

作　　者：斯妍娜[1] 鲍红光[1] 韩流[1] 徐丽[1] 王绪林[1] 沈妍[1] 

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)麻醉科,210006

出　　处：《中华麻醉学杂志》2012年第3期301-303,共3页Chinese Journal of Anesthesiology

基　　金：基金项目：南京医科大学科技发展基金项目（2010NJMU052）;南京市医学科技发展项目（201108028）

摘　　要：目的评价右美托咪啶预先给药和后处理对大鼠肾缺血再灌注损伤的影响。方法健康雄性Wistar大鼠32只，体重220～300g，3—4月龄，采用随机数字表法，将大鼠随机分为4组（n=8）：假手术组（s组）、缺血再灌注组（UR组）、右美托咪啶预先给药组（Pre组）和右美托咪啶后处理组（Post组）。肾缺血60min，再灌注48h制备肾缺血再灌注损伤模型。Pre组和Post组分别于缺血前30min和再灌注开始时腹腔注射右美托咪啶50ug／kg。于再灌注即刻、24、48h（T1-3）时分别测定血清肌酐和血尿素的浓度。再灌注结束时取肾组织，光镜下观察病理学改变，并行急性肾小管坏死评分，采用TUNEL法测定细胞凋亡，计算细胞凋亡指数。结果与s组比较，其余3组T2，时血清肌酐和血尿素的浓度升高，急性肾小管坏死评分和细胞凋亡指数升高（P〈0．05）。与I／R组比较，Pre组和Post组己，时血清肌酐和血尿素浓度降低，急性肾小管坏死评分和细胞凋亡指数降低（P〈0．05）。结论右美托咪啶预先给药和后处理均可减轻肾缺血再灌注损伤，其机制与抗细胞凋亡作用有关。Objective To investigate the effects of dexmedetomidiue pretreatment and postconditioning on renal ischemia-reperfusion （I/R） injury in rats. Methods Thirty-two male Wistar rats, aged 3-4 months, weighing 220-300 g, were randomly divided into 4 groups （n = 8 each）： sham operation group （group S）, I/R group, dexmedetomidine pretreatment group （group Pre） and dexmedetomidine postconditioning group （group Post）. The rats were anesthetized with phenobarbital sodium 65 mg/kg. Renal I/R was produced by occlusion of both renal pedicles for 60 rain followed by 48 h reperfusion. Dexmedetomidine 50 ug/kg was given intraperitoneally at 30 min before ischemia and at the beginning of reperfusion in Pre and Post groups respectively. The concentrations of serum creatinine and blood urea were determined at 0, 24 and 48 h of reperfusion （T1-3） . The renal tissues were ob- tained at the end of reperfusion for microscopic examination and detection of apoptosis by TUNEL assay. Acute kidney tubular necrosis was scored and apoptosis index （AI） was calctdated. Results Compared with group S, the concentration of serum creatinine and blood urea at T2.3, and acute kidney tubular necrosis score and AI were sig- nificantly increased in I/R, Pre and Post groups （P 〈 0.05）. Compared with I/R group, the concentration of se- rum creatinine and blood urea at T2.3, acute kidney tubular necrosis score and AI were significantly decreased in Pre and Post groups （ P 〈 0.05）. Conclusion Both dexmedetomidine pretreatment and postconditioning can attenuate renal I/R injury through inhibition of cell apoptosis in rats.

关 键 词：右美托咪啶 再灌注损伤 肾疾病 

分 类 号：R96[医药卫生—药理学]