生物活性炭纤维小球对养殖池塘处理及除氮优势菌的鉴定研究  

REMOVAL OF AMMONIA NITROGEN IN POND WATER ACF BEADS IMMOBILIZED WITH MICROBE AND IDENTIFICATION OF PREDOMINANT DEGRADATION FUNGUS

作　　者：尹艳娥[1] 鲁超[1] 徐亚岩[1] 康伟[1] 王翠华[1] 徐兆礼[1] 

机构地区：[1]农业部东海与远洋渔业资源开发利用重点实验室,中国水产科学研究院东海水产研究所,上海200090

出　　处：《水处理技术》2012年第6期18-21,共4页Technology of Water Treatment

基　　金：中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)资助项目(2007T01)

摘　　要：采用生物活性炭纤维小球处理养殖池塘水体中氨氮,研究了不同浓度的氨氮去除情况。结果表明:在1～7 d,5、25、50 mg/L的氨氮去除效果均呈上升趋势,最佳去除率分别达到了85.4%、79.5%、76.7%;在8～14 d,对氨氮的去除效果均处于一个波动过程,但在14 d内,去除率分别保持在74%、65%、63%以上;在15～21 d对氨氮的去除效果均呈下降趋势。生物活性炭纤维小球在处理氨氮过程中亚硝酸盐氮和硝酸盐氮也是处于变化的动态过程。通过多次反复筛选,从生物活性炭纤维小球中筛选出对氨氮具有较强去除效能的优势菌。通过对菌株的个体形态特征、菌落形态特征进行观察,最终确定2种优势菌为硝化菌和恶臭假单胞菌。对2种优势菌的生态影响因子研究表明,最适宜的温度为25～35℃,适宜的pH为中性及偏碱性。在相同的环境条件下,硝化菌对氨氮的去除效果优于恶臭假单胞菌。Ammonia nitrogen in pond water was removed by ACF beads immobilized with microbe. The fimction of ACF beads immobilized with microbe was analyzed according to the removal efficiency of ammonia nitrogen. The results indicated when the initial concentration of ammonia nitrogen was respectively 5 mg/L, 25 mg/L and 50 mg/L in the simulated pond, in 1 -7 d, the removal efficiency of ammonia nitrogen gradually increased to 85.4%, 79.5%and the removal efficiency of ammonia nitrogen was degressive. And the concentration of nitrate nitrogen and nitrite nitrogen were also variational with variation of ammonia nitrogen. The predominant fungus that had good ability to remove ammonia nitrogen was isolated from the ACF beads immobilized with microbe. These fungus were observed from morphologic characteristics and biochemical properties. Finally, two predominant fungus, Nitrifying bacteria and Pseudomonas putida, were confirmed. The factors affecting two predominant fungus were also discussed. The results indicated that the most suitable pH value and temperature for nitrification were 7- 8 and 25 -35℃, respectively. And in the same condition for pH value and temperature, the removal efficiency of nitrifying bacteria was better than pseudomonas putida.

关 键 词：生物活性炭纤维 无机氮 优势降解菌 

分 类 号：X703.1[环境科学与工程—环境工程]