聚丁二炔纳米囊泡生物传感器比色检测空肠弯曲菌的初步研究  

A Polydiacetylene Vesicle-based Colorimetric Detection of Campylobacter jejuni Using a DNA Aptamer as Recognition Element

作　　者：董建涛[1] 欧阳后先[1] 徐柯[1] 吴文鹤[1] 吕建新[1] 

机构地区：[1]温州医学院检验医学省部共建教育部重点实验室,温州325035

出　　处：《科技通报》2012年第5期75-79,共5页Bulletin of Science and Technology

基　　金：国家自然科学基金项目(81101148);温州市科技计划重大项目(Y20080080);浙江省科技计划面上科研项目(2009C33036);浙江省新苗人才计划(2010R413003)

摘　　要：本实验构建一种可以快速比色检测空肠弯曲菌的聚丁二炔纳米囊泡生物传感器。适配体通过化学反应耦合到聚丁二炔纳米囊泡表面,完成对囊泡的功能化。适配体与空肠弯曲菌特异识别,引起纳米囊泡溶液吸收光谱的改变,通过比色响应值衡量检测体系中有无空肠弯曲菌。该方法特异性好,与PCR对比实验相符;操作简便,检测时间短,仅需30 min。A polydiacetylene vesicle-based biosensor for rapid colorimetric detection of Campylobacter jejuni was devel- oped in this work. Aptamers coupled to the surface of polydiacetylene nanovesicle by chemical reaction in order to achieve vesicle functionalization. Molecular recognition between Campylobacter jejuni and aptamers on the surface of the vesicle lead to absorption spectrum transition of polydiacetylene by which quantitative detection of Campylobacter jejuni could be realized. This method can detect Campylobacter jejuni in 30 mins with high specificity and simple operation.

关 键 词：纳米囊泡 比色检测 空肠弯曲菌 适配体 

分 类 号：R446.5[医药卫生—诊断学]