重组白细胞介素2生物检定的改良MTT比色法  

MODIFIED MTT COLORIMETRY FOR DETERMINATION OF RECOMBINANT INTERLEUKIN 2 ACTIVITY

作　　者：焦忠义 沈丽娟 李晓嵘 朱建伟 沈优君 王新力[1] 李厉 

机构地区：[1]上海医科大学放射医学研究所,上海200032 [2]上海医药工业研究院,上海200040

出　　处：《中国医药工业杂志》1990年第3期133-135,共3页Chinese Journal of Pharmaceuticals

摘　　要：改良的MTT比色法用10％SDS-5％异丁醇-0.01mol/L HCl溶解CTLL_2细胞内的甲?,得到稳定的甲?溶液,其0D_(570)值能保持几十小时基本不变,便于比色分析。测定重组白细胞介素2的结果与~3H-TdR掺入法大致相同,两者的相关系数为0.991(p<0.01)。In the improved colorimetry for recombinant interleukin-2 (γIL-2), the formazan in mitochondria of CTLL_2 cells is dissolved in 10% SDS-5%isobutanol-0.01 mol/L HCl to offer a more stable purple solution with OD_(570)value hardly changing at least a few days. The correlation coefficient betweenthe results of this method and ~3H-TdR incorporation method is 0.991 (p<0.01).

关 键 词：白细胞介素 MTT比色法 生物检定 

分 类 号：TQ460.72[医药卫生—药物分析学]