海南吊罗山原始林区抗香蕉枯萎病土壤放线菌的分离及田间防治效果试验  

Isolation of Actinomycetes from Diaoluo Mountain and Control Effects Test Against Banana Wilt in the Field

作　　者：茹祥[1] 曾涛[2] 莫坤联[1] 陈海洋[1] 陈汉清[2] 曾会才[2,3] 

机构地区：[1]海南大学环境与植物保护学院,海口570228 [2]中国热带农业科学院热带生物技术研究所/热带作物遗传资源与改良农业部重点开放实验室,海口571101 [3]中国热带农业科学院海口实验站/香蕉研究所,海口570102

出　　处：《中国农学通报》2012年第13期97-102,共6页Chinese Agricultural Science Bulletin

基　　金：公益性行业(农业)科研专项课题"尖孢镰刀菌共性致病机理及香蕉枯萎病生防与综合防控研究"(200903049-2);国家973计划前期研究专项课题"海南热带雨林土壤微生物抗香蕉枯萎病菌活性成分的研究"(2009CB12904);海南大学"211工程"建设项目;海南大学环境与植物保护学院研究生创新平台

摘　　要：为了从热带原始雨林土壤中筛选得到抗香蕉枯萎病的高效生防放线菌。采用平板对峙培养,盆栽防效和田间防效试验等方法,从海南吊罗山原始林区土壤样品分离的385株放线菌中筛选出了16株对香蕉枯萎病菌具有拮抗活性的放线菌,其中DL-28和DL-24菌株具强抑菌活性。盆栽防治试验结果表明:接种100天后,菌株DL-28和DL-24对香蕉枯萎病的平均防治效果分别达83.65%和76.51%。田间防治试验结果表明;香蕉定植7个月后,菌株DL-28和DL-24对香蕉枯萎病的平均防效达46.5%和60%。In order to sift Actinomyces efficiently against Fusarium oxysporum f. sp. Cubense from virgin forest, antagonistic assay in plates, potted control effect test and field trials were conducted. 385 strains of Actinomyces were isolated from soil samples which were collected from Diaoluo Mountain virgin forest, 16 strains of these Actinomyces were proved to have resistance to Fusarium oxysporum f. sp. Cubense, and strains DL-28 and DL-24 had prominent bioactivity. The results of potted control effect test showed that the average control efficacy of DL-28 and DL-24 against Fusarium oxysporum f. sp. cubense was 83.65% and 76.51% . And result of field trials demonstrated that after the bananas were planted 7 months, the average control efficacy of strains DL-28 and DL-24 against Fusarium oxysporum f. sp. cubense was 46.5% and 60%.

关 键 词：香蕉枯萎病 放线菌 拮抗 田间试验 

分 类 号：Q939.9[生物学—微生物学]