栽培桃与野生桃果实发育中山梨醇含量及其相关酶活性的变化  

Changes in Sorbitol Content and Its Related Enzyme Activities Between ‘Jiubao’and Wild Peach During Peach Fruit Development

作　　者：王新月[1] 关伟[2] 赵晓萌[1] 刘悦萍[1] 

机构地区：[1]北京农学院生物技术学院,北京102206 [2]北京市平谷区人民政府果品办公室,北京101200

出　　处：《中国农学通报》2012年第13期185-189,共5页Chinese Agricultural Science Bulletin

基　　金：北京市科技新星计划"桃果核发育及裂核机理研究"(2008B22);北京市属市管高校人才强教计划资助项目"裂核桃果实内果皮发育的解剖学特点及特异蛋白表达分析"

摘　　要：试验研究桃果实发育过程中,山梨醇含量及其相关酶活性的变化规律,探究其与裂核现象的可能关系。以栽培桃‘久保’和野生桃为试验材料,测定桃果实不同发育时期中果皮和内果皮的山梨醇含量以及与山梨醇代谢相关酶——山梨醇脱氢酶(SDH)和山梨醇氧化酶(SOX)活性的变化。在果实发育的第1个时期,‘久保’中果皮山梨醇含量整体趋势高于野生桃,SDH和SOX的活性低于野生桃,说明在幼果生长期,‘久保’中果皮内的山梨醇代谢较野生桃弱。在果实发育的第2个时期,对比‘久保’与野生桃内果皮,‘久保’的山梨醇含量高于野生桃,SDH的活性低于野生桃,说明在桃果实硬核期,野生桃内有较强的山梨醇代谢活性,从而有利于果糖的形成,为桃果核的木质化提供能量来源。在果实发育的第3个时期,‘久保’中果皮内的山梨醇含量比野生桃要上升的快,表明‘久保’果实可能更多地利用外源糖供给,促进果实的迅速膨大。This experiment was carried out to study changes in sorbitol content and its related enzyme activities during peach （Amygdalus persica） fruit development, in order to find any relationship between sorbitol and splitting. The contents of sorbitol and the activities of sorbitol-metabolizing enzymes, including sorbitoldehydrogenase （SDH）, sorbitoloxidase （SOX） were studied in different development of wild peach and cultivating peach‘Jiubao’. At first period of fruit development, the content of sorbitol in mesocarp of wild peach was higher than that of‘Jiubao’, the activities of SDH and SOX were the same. In second period of fruit development, the content of sorbitol in endocarp of wild peach was higher than that of‘Jiubao’, but the activities of enzyme was opposite. In this period, the metabolism of sorbitol was more active to benefit the hardening of endocarp. In the third period of development, the content of sorbitol in mesocarp of‘Jiubao’was higher; it showed the‘Jiubao’fruit depend on external sugar source to develop until maturity.

关 键 词：桃 山梨醇 酶活性 

分 类 号：S662[农业科学—果树学]