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作 者:葛安静[1] 张春华[2] 董清华[1] 赵密珍[2] 宋长年[3] 张希[1]
机构地区:[1]北京农学院植物科学技术学院,北京102206 [2]江苏省农业科学院园艺研究所,南京210014 [3]南京农业大学园艺学院,南京210095
出 处:《中国农学通报》2012年第13期215-220,共6页Chinese Agricultural Science Bulletin
基 金:北京市教委科技发展计划项目(KM200710020012)
摘 要:为了研究草莓SBP基因家族的功能,利用多种生物信息学软件,对SBP蛋白序列的系统发生和基因组定位进行分析,对其氨基酸组成成分、理化性质以及二、三级结构进行分析和预测等。结果表明草莓16条SBP蛋白序列与拟南芥16条SBP蛋白序列一起分成了3个亚族,说明拟南芥与草莓SBP基因间具有较高的保守性。基因组定位发现16个SBP基因分布在6条染色体上。研究还发现不同亚族间氨基酸数目、氨基酸序列间的疏水性存在一定的差异,而它们的二级结构主要以随机卷曲为主,三级结构相似。SBP基因家族在植物的生长、发育等多个方面起重要作用,笔者实验结果为草莓SBP基因家族的进一步功能分析提供了重要研究基础。The aim was to study the function of SBP gene family in strawberry. Various bioinformatics methods were used to analyze the phylogenesis of SBP protein sequences and the localization of SBP genome, their amino acid composition, physical and chemical characteristics, as well as secondary and tertiary structures. The result showed that these 16 amino acid sequences could be clustered into 3 groups together with 16 SBP sequences of Arabidopsis thaliana, which illuminated that SBP genes had highly conservative between strawberry and Arabidopsis thaliana. Further genetic mapping of SBP genome localization found that they could be distributed on 6 chromosomes. The research still found that the number of amino acid and hydrophobic of amino acid sequences in different subfamilies presented some differences, however, the results of secondary structure predicted that the main composition of 16 amino acid sequences was random curl and the tertiary structure of all the sequences were quite similar. SBP gene family plays great role in plant growth and development. The results of this investigation could definitely provide a significant foundation for further research on function analysis of SBP gene family in Strawberry.
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