酒精培养C3A细胞NOTCH信号通路的表达  

Notch signaling pathway expression in C3A cell line cultured with ethanol

作　　者：谢俏[1] 李汇华[2] 李鑫[1] 徐有青[1] 

机构地区：[1]首都医科大学附属北京天坛医院消化内科,北京市100050 [2]首都医科大学附属北京天坛医院病理教研室,北京市100050

出　　处：《实用肝脏病杂志》2012年第3期203-205,共3页Journal of Practical Hepatology

基　　金：北京市自然科学基金资助项目(编号:7112048)

摘　　要：目的观察Notch信号通路在酒精性肝病体外模型中的表达情况及其对C3A细胞增殖的影响。方法将人CYP2E1目的基因转染到C3A细胞系,筛选出稳定表达CYP2E1的肝细胞系为实验组。同时用空质粒转染C3A细胞系为对照组。两组细胞培养基中均加入等浓度酒精、等体积灭菌水和Notch抑制剂进行培养,采用MTT法检测细胞存活率,采用Western-blot法检测Notch下游蛋白Notch受体胞内区域(NICD1)和Hairy/Enhancer ofSplit蛋白(HES1)的表达,采用RT-PCR法检测Notch1mRNA水平。结果酒精使两组细胞存活率下降,Notch抑制剂提高了实验组细胞的存活率;实验组细胞NICD1和HES1表达高于对照组,加入Notch抑制剂后两者表达明显下降;实验组细胞Notch1mRNA水平高于对照组。结论酒精可以激活C3A细胞的Notch信号通路,Notch信号通路阻断剂可使酒精处理的肝细胞存活率升高。Objective To study the expression of Notch signaling pathway influenced by ethanol in C3A cells in vitro. Methods C3A cells were served as the control group,and the CYP2E1 cells were as experiment group. Equal concentration of ethanol and Notch signaling pathway inhibitor were added to the two groups of cells. RT-PCR and Western blotting were used to testify the expression of Notchl,NICD1 and HESl,respectively. MTY assay was used to evaluate the viability of cells. Results The cell viability in the two groups was decreased by ethanol,while the Notch signaling pathway inhibitor improved the viability of cells; The expression of NICD1 and HES1 was up-regulated in the experimental group,while when Notch signaling inhibitor was added into the medium,the expression of NICD1 and HES1 was significantly down-regulated; The Notchl mRNA level was also up-regulated in the experimental group. Conclusion Ethanol can activate the expression of Notch signaling pathway in C3A models.

关 键 词：C3A细胞 酒精 NOTCH信号通路 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]