丝裂霉素产生菌丛生链霉菌的靶向育种  

TARGET BREEDING OF MITOMYCIN PRODUCING STRAIN OF STREPTOMYCES CAESPITOSUS

作　　者：宋友礼[1] 许美云[1] 孙人杰 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《中国医药工业杂志》1990年第6期244-248,共5页Chinese Journal of Pharmaceuticals

摘　　要：以丝裂霉素产生菌丛生链霉菌638及其衍生菌株作出发菌株,用UV诱变再经过易出误差修复和建立在丝裂霉素生物合成途径基础上的靶问育种新技术,得到许多突变型菌株。精氨酸抗性突变型菌株14-39孢子形成延迟,产生的丝裂霉素效价比原始菌株638提高7倍,丝裂霉素提取得量最高可达82mg/L。Mitomycin producing strain Streptomyces caespitosus 638 and its derived strains were used as the starting strains, many mutants such as citrulline-, methionine-component mutants and arginine-resistant mutant were obtained by UV induced mutation and error prone repair and target breening based on mi- tomycin biosynthesis. Arginine-resistant mutant strain 14-39 were characterized by the delayed sporulation, and the potencies of mitomycin increased by 7-fold vs. the original strain 638, to reach 82mg/L of mitomycin C.

关 键 词：丝裂霉素 丛生链霉菌 靶向育种 

分 类 号：TQ465.92[化学工程—制药化工]