维生素D制剂的HPLC测定法  

HPLC DETERMINATION OF VITAMIN D PREPARATION

作　　者：张怡[1] 杨岗[1] 丁寅芬[1] 陈如进[1] 

机构地区：[1]福建省药品检验所,福州350000

出　　处：《中国医药工业杂志》1990年第6期256-261,共6页Chinese Journal of Pharmaceuticals

摘　　要：采用加热过的VD_3溶液以2支主波长分别为254及365nm的8w紫外灯照射5min后校验分析柱系统的适用性,可用正相HPLC(Waters Resolve Silica柱,0.3％正戊醇的正己烷溶液为流动相,254nm检测)分离VD的6个异构体,分离度均大于1.0。在0.5～60u范围内VD_3呈线性。针对性地调节净化往系统可用内标法(邻苯二甲酸二甲酯)或外标法(热平衡法)测定低浓度(1ppm)制剂,误差±10％。高浓度制剂测定误差为±3％。In the column system suitability test following irradiation of heated VD_3 solution by UV with main wave length 254 and 395nm for 5 min, six isomers formed from VD could be separated with the norma1 phase HPLC (Wa- ters Resolve Silica column, 0.3% n-pentanol in hexane as mobile phase, detected at 254nm), with resolution factor, R, greater than 1.0, The linearity was obtained in 0.5～60 u VD_3. The low concentration (1 ppm) preparation could be determined by internal (dimethyl phthalate) method or external (thermal equilibrium) me- thod with error±10%. The error in determination of high concentration prepara- tion was ±3%.

关 键 词：维生素D 高效液相色谱 

分 类 号：TQ460.72[医药卫生—药物分析学]