通腑颗粒质量标准研究  

Study on Quality Standard of Tongfu Granule

作　　者：丁红梓[1] 弓晓东[2] 谈瑄忠[3] 毛春芹[2] 陆兔林[2] 

机构地区：[1]南京市胸科医院,江苏南京210029 [2]南京中医药大学药学院,江苏南京210046 [3]南京中医药大学第三附属医院,江苏南京210001

出　　处：《中国中医药信息杂志》2012年第6期57-59,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立通腑颗粒的质量标准。方法采用薄层色谱法对方中的大黄、枳实、厚朴进行定性鉴别,用高效液相色谱法对芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进行含量测定。结果薄层色谱斑点清晰,分离度良好,阴性对照无干扰。芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.038～0.610、0.048～0.780、0.068～1.076、0.056～0.904、0.022～0.336μg范围内与峰面积积分值呈良好的线性关系,平均回收率依次为96.01%、94.67%、100.94%、90.06%、92.37%,RSD分别为1.90%、2.77%、2.18%、1.88%、2.84%。结论定性定量方法简便、结果准确可靠,所建立的标准可用于通腑颗粒的质量控制。Objective To establish the quality standard of Tongfu granule. Methods TLC was used for the qualitative identification of Rheum palmatum L., Citrus aurantium L. and Magnolia officinalis. HPLC was used to determine the content of aloe-emodin, rhein, emodin, chrysophanol and physcion. Results TLC spots were clear and weU-separated without negative interference. The linear range of aloe-emodin, rhein, emodin, chrysophanol and physcion was 0.038--0.610, 0.048--0.780, 0.068--1.076, 0.056--0.904 and 0.022--0.336μg, respectively. The average recovery was 96.01%, 94.67%, 100.94%, 90.06% and 92.37%, respectively. RSD was 1.90%, 2.77%, 2.18%, 1.88% and 2.84%, respectively. Conclusion The method is simple, accurate and reliable with good precision. It can be used for the quality control of Tongfu granule.

关 键 词：通腑颗粒 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 薄层色谱法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]