通过改良农杆菌介导的floral-dip法和花粉管通道法转化紫薇  

Transformation of Lagerstroemia indica by improved Agrobacterium-mediated floral-dip and pollen-tube pathway method

作　　者：陈彦[1] 孙宽莹[2] 张涛[2] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059 [2]聊城大学农学院,山东聊城252059

出　　处：《浙江大学学报（农业与生命科学版）》2012年第3期250-255,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：山东省自然科学基金资助项目(Y2008D41)

摘　　要：以绿色荧光蛋白(GFP)基因为报告基因,分别用改良农杆菌介导的花序浸渍法(floral-dip)和花粉管通道法转化紫薇(Lagerstroemia indica),统计果实数、结实率、成苗率,然后用紫外灯照射28d左右的幼苗筛选转化株。结果表明:花粉管通道法转化紫薇的结实率、成苗率均明显高于floral-dip法;用手携式紫外灯(365nm)照射进行活体鉴定,floral-dip法转化率比花粉管通道转基因法转化率高0.06%,但未达到显著水平;而花粉管通道法的结实率比floral-dip法的高11.87%～26.74%,更利于获得转化植株。说明通过花粉管通道法进行转化更适合于紫薇转基因新品种的培育。Lagerstroemia indica was transformed by an improved Agrobacterium-mediated floral-dip and pollen-tube pathway method, respectively, using green fluorescent protein gene （GFP） as a report gene, and its fruit number, rate of seed-setting set and seedling rate were counted. The transformed plants were screened from the 28-day seedlings irradiated by a portable ultraviolet lamp at 365 nm. The experimental results showed that the rate of seed-setting set and seedling rate by pollen-tube pathway method were significantly higher than those by the floral-dip method. The transformation rate of L. indica by＇ the floral-dip method was higher by 0.06% than that by the pollen-tube pathway, which didn＇t yet reach significant levels. The rate of seed-setting set by the pollen-tube pathway method was higher by 11.87%-26.74% than that by the floral-dip method, indicating that the pollen-tube pathway method is more beneficial for transformation of L. indica.

关 键 词：紫薇 改良农杆菌介导的floral—dip法 花粉管通道法 转化 

分 类 号：Q812[生物学—生物工程] S685.99[农业科学—观赏园艺]