基于辅因子调控对大肠杆菌两阶段发酵产丁二酸的影响  

Effect of cofactor regulation on succinate production in Escherichia coli during dual-phase fermentation

作　　者：刘嵘明[1] 梁丽亚[1] 王光明[1] 张敏[1] 马江锋[1] 陈可泉[1] 姜岷[1] 韦萍[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009

出　　处：《生物加工过程》2012年第3期1-5,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(21076105);国家重点基础研究发展计划(973计划)资助项目(2009CB724701)

摘　　要：考察了E.coli NZN111及其重组菌株E.coli NZN111/pTrc99a-pncB发酵生产丁二酸的性能。E.coli NZN111两阶段发酵丁二酸的同时,会造成丙酮酸的大量积累。研究发现:通过过量表达烟酸转磷酸核糖激酶,两阶段发酵重组菌株E.coli NZN111/pTrc99a-pncB,减少丙酮酸的积累且无副产物乙酸生成,提高丁二酸的产量,丁二酸得率和耗糖速率分别提高了139%和20%。Two strains, including E. coli NZN111 and its recombinant strain E. coli NZN111/pTrc99apncB were investigated as the production of succinate. During dual-phase fermentation, E. eoli NZN111 generated succinate as the main products, meanwhile, pyruvate was accumulated. With the overexpressed the nicotinic acid phosphoribosyl transferase, the recombinant strain lowered the concerntration of pyruvate, eliminated the generation of acetate and increased the production of succinate under dual-phase fermentation. The yield of succinate and the glucose consumption rate was increased by 139%, 20%, respectively.

关 键 词：大肠杆菌 烟酸 转磷酸核糖激酶 丁二酸 

分 类 号：Q815[生物学—生物工程]