内蒙古河套灌区湿地芦苇根际可培养细菌多样性  

Diversity of Culturable Rhizosphere Bacteria from Reed in Hetao Irrigation Area,Inner Mongolian Autonomous Region

作　　者：孙磊[1] 刘莹[1,2] 姚娜[2] 邵红[1] 李潞滨[2] 

机构地区：[1]河北大学生命科学学院,河北省微生物多样性研究与应用实验室,河北保定071002 [2]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091

出　　处：《湿地科学》2012年第2期176-180,共5页Wetland Science

基　　金：国家水体污染控制与治理科技重大专项项目(2009ZX07212-004);河北省自然科学基金项目(C2009000180);河北大学引进人才专项基金项目(2006-088);中央级公益性科研院所基本科研业务费专项资金项目(RIF2010-16);引进国际先进林业科学技术项目(2011-4-71)资助

摘　　要：以内蒙古河套灌区湿地植物芦苇(Phragmites australis)根际土壤为材料,采用分离培养法对天然湿地和退化湿地的芦苇根际细菌多样性进行了研究。16SrRNA基因系统发育分析的结果显示,分离自天然湿地芦苇根际的45株细菌分属于γ-变形菌纲(44.44%)、厚壁菌门(Firmicutes)(42.22%)和放线菌门(Actinobacteria)(13.33%)3大类群,其中,γ-变形菌纲为最优势类群,芽孢杆菌属(Bacillus)(42.2%)为最优势菌属;分离自退化湿地芦苇根际的44株细菌分属γ-变形菌纲(13.63%)、β-变形菌纲(6.82%)、厚壁菌门(47.73%)及放线菌门(Actino-bacteria)(31.82%)4大类群,其中厚壁菌门为最优势类群,芽孢杆菌属(40.9%)为最优势菌属。研究结果表明,天然湿地和退化湿地芦苇根际细菌都具有较丰富的多样性,但天然湿地与退化湿地芦苇根际细菌群落结构存在一定差异。The diversity of culturable bacteria of reed （Phragmites australis） rhizosphere from natural and degraded wetlands in Hetao irrigation region of Inner Mongolia Autonomous Region was investigated in this study. A total of 99 bacterial strains with different colony characteristics were isolated on R2A medium： 45 isolates from natural wetlands, 44 isolates from degraded wetlands. Population densities of rhizosphere bacteria were （1.7±0.14）×108 cfu/gfw （colony-forming units per gram fresh weight） for natural wetlands, and （1.3±0.38）× 108 cfu/gfw for de- graded wetlands. Bases on 16S rRNA gene sequence analysis, 45 rhizosphere isolated from natural wetlands were grouped into 3 groups, Gammaproteobacteria （44.44%）, Firmicutes （42.22%）, and Actinobacteria （13.33%）. The dominant group was Gammaproteobacteria. All bacterial isolated from natural wetlands were assigned to 9 genera. The dominant genus was Bacillus （42.22%） and Pseudorrumas （22.2%）; 44 rhizosphere isolates from degraded wet- lands were belonged to 4 groups, Gammaproteobacteria （13.64%）, Betaproteobacteria （6.82%）, Firmicutes （47.73%）, and Actinobacteria （31.82%）. The dominant group was Firmicutes. All bacterial isolates from degraded wetlands were assigned to 13 genera, and the genus Bacillus （40.91%） was the predominant genus, which included 18 bacterial isolates. The results indicated that the diversity of rhizosphere bacteria from the wetlands in study area was abundant and the rhizosphere bacterial communities between natural and the degraded wetlands had differences.

关 键 词：湿地 芦苇 根际细菌 多样性 16S RRNA基因 

分 类 号：Q938[生物学—微生物学]