水芹(Oenanthe javaica)浸出液对小球藻(Chlorella vulgaris)生长及超微结构的影响  

Effects of Oenanthe javaica Extracts on Growth and Ultrastructure of Chlorella vulgaris

作　　者：袁亚光[1] 李思宇[1] 宰学明[2] 钦佩[1] 

机构地区：[1]南京大学盐生植物实验室,江苏南京210093 [2]金陵科技学院园艺学院,江苏南京210038

出　　处：《生态与农村环境学报》2012年第3期266-270,共5页Journal of Ecology and Rural Environment

基　　金：国家林业公益性行业科研专项(200904001)

摘　　要：采用在不同浓度水芹(Oenanthe javaica)浸出液中纯培养小球藻(Chlorella vulgaris)的方法,研究水芹浸出液对小球藻细胞数量、叶绿素含量和藻细胞超微结构的影响。结果显示,10 g.L-1水芹浸出液对小球藻的生长和叶绿素含量具有明显的促进作用;20 g.L-1水芹浸出液处理组藻细胞数量和叶绿素含量增加持续至第7天,但增幅低于对照组,7 d后抑制作用增强;高浓度(30～50 g.L-1)水芹浸出液对小球藻细胞数量和叶绿素含量的抑制作用在第5天开始变得显著,并随时间延长而加剧,具有浓度效应;经40 g.L-1水芹浸出液处理后,小球藻细胞壁断裂甚至消失,细胞中叶绿体片层肿胀甚至解体,核膜破裂,核质外渗。结果表明水芹浸出液对小球藻具有化感效应,总体呈现低浓度促进、高浓度抑制的规律。Pure cuhure of chIorella was done in solutions different in concentration of Oenanthe javaica extracts added, to explore effects of O. javaica extracts on growth, chlorophyll content and ultrastructure of chlorella. Results show that in the treatment of 10 g ·L^-10. javaica extract growth of chlorella was significantly promoted, and in the treatment of 20 g ·L^-1. javaica extract the number of ceils and chlorophyll content of chlorella increased with a margin narrower than that in the control in the first 7 days, and afterwards, the growth was inhibited. In the treatments of 30 - 50 g ·L^-10. javaica extract, phenomenon of the inhibition became obvious on the fifth day and more obvious with the time going on and with the increasing concentration of the extract added as well. In the treatment of 40 g ·L^-10. javaica extract, cell walls of the algae broke up and even vanished, chloroplast lamellas in the cells swelled and even disintegrated, and karyolem- mas ripped with karyoplasm extravasating. The findings indicate that 0. javaica extract had an allelopathic effect on chlo- rella, and when low in concentration, it promotes growth of the algae, but when high, it acts reversely.

关 键 词：水芹 浸出液 小球藻 化感作用 叶绿素 超微结构 

分 类 号：Q178.1[生物学—水生生物学]