BXSB小鼠血清抗DNA抗体水平及其对淋巴细胞转化的影响  被引量:2

The level of the anti-DNA antibody in the serum of BXSB mouse and the effect on lymphocyte proliferation

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作  者:梅林[1] 汪静雪[1] 邓鸿业[2] 丁桂凤[2] 范少光[1] 

机构地区:[1]北京医科大学生理学系 [2]北京医科大学免疫学系,北京100083

出  处:《基础医学与临床》2000年第1期34-37,共4页Basic and Clinical Medicine

基  金:卫生部基金!No.95-2-23

摘  要:用酶联免疫吸附剂测定法(ELISA)和3H-胸腺嘧啶核苷掺入法,观察一种自发性系统性红斑狼疮(SLE)好发小鼠(BXSB小鼠)在不同月龄时,血中抗DNA抗体水平的变化,并观察该血清对正常LACA小鼠淋巴细胞转化的影响。结果显示,自3月龄起,BXSB雄性鼠血中的抗DNA抗体水平开始升高,4月龄上升至高峰,5月龄仍保持较高水平,各月龄BXSB血清与对照血清相比,P<0.05(ANOVA)。同时,该血清对正常LACA小鼠淋巴细胞转化也有抑制作用,以4月龄血清的抑制作用最大。如果将同一只小鼠血清抗DNA抗体水平的变化与该血清对淋巴细胞转化的影响做回归和相关分析,发现两者有明显的正相关关系(,0.232,<一53,尸<O.05)。提示雄性*x8B小鼠可能从3月龄开始表现SLE。发病后,血清中产生某种能够抑制淋巴细胞转化的物质,它的产生与抗DNA抗体有平行关系。The level of the anti-DNA antibody in BXSB serum and the effect of BXSB serum on lymphocyte proliferation in normal mouse were observed by using enzyme-linked immunosorbent assay(ELISA) and 3H -thymidine incorpora- tion. The level of anti-DNA antibody in male BXSB serum rose from the age of 3 month and reached to the highest value at the age of 4 month. Although there were a little drop in the antibody level at the age of 5 month, it was still higher than the one of control group (P=0.032, BXSB serum vs control serum, ANOVA ). The experiment also showed that there was a dramatic negative correlation between the level of anti-DNA antibody and the effect of the same portion of BXSB serum on normal lymphocyte proliferation(r=0.232, n=53, P<0.05 ). It was concluded that the male BXSB mouse may manifest SLE from the 3rd month after birth. There generated in the SLE serum some substances which not only showed the inhibitory effect on normal lymphocyte proliferation but also were parallel, in its effect, with the level of anti-DNA antibody.

关 键 词:系统性红斑狼疮 DNA抗体 淋巴细胞转化 

分 类 号:R331.1[医药卫生—人体生理学]

 

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