抗成骨肉瘤单克隆抗体的研制及其反应性  

MONOCLONAL ANTIBODIES AGAINST HUMAN OSTEOSARCOMA AND THEIR CHARACTERISTICS

作　　者：刘全海[1] 孟德鸣[1] 冯怡[1] 陈瑞岳 胡士亮 李可[1] 郭荻萍[2] 虞建邦 

机构地区：[1]上海医药工业研究院药理室,上海200433 [2]上海铁道医学院 [3]上海第一人民医院

出　　处：《中国医药工业杂志》1990年第10期436-439,共4页Chinese Journal of Pharmaceuticals

摘　　要：用人新鲜骨肉瘤组织免疫BALB/C小鼠,经融合、筛选,获4株杂交瘤细胞株,分泌的单克隆抗体AOS-01、02、03、及04与多种人体正常组织无交叉反应。用ELISA、免疫荧光或免疫组织化学染色法检测了该4株单抗与3株人体骨肉瘤细胞株及10例人骨肉瘤标本的反应性,结果4株单抗对不同标本的反应性不相同。Spleen cells of BALB/C mice immunized with fresh human osteo-sarcoma cells were fused with mouse myeloma cell line, NSO. The four mono-clonal antibodies obtained did not cross-react with various human normal tissuesusing ELISA, immunofluorescence and immunohistochemical staining. But reac-tions of the 4 monoclonal antibodies with 3 human osteosarcoma cell lines and 10clinical samples were different.

关 键 词：单克隆抗体 骨肉瘤 研制 反应性 

分 类 号：R392－33[医药卫生—免疫学]