犬巴贝西虫病PCR检测方法的建立  

Establishment of A PCR Assay for Detecting Canine Babesia

作　　者：尚泽松[1] 李小康[1] 王雪莹[1] 张才[1] 江丰伟[1] 赵艳辉[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003

出　　处：《河南农业科学》2012年第5期158-160,共3页Journal of Henan Agricultural Sciences

基　　金：河南科技大学SRTP项目(2009102;2010244)

摘　　要：为快速、准确检测犬巴贝西虫病,根据GenBank中收录的犬的巴贝西虫18sRNA基因序列,设计合成1对特异性诊断引物,建立了PCR诊断方法。结果扩增出了犬巴贝西虫18sRNA基因的一段大小为319bp片段,经测序,所扩增序列与报道的序列完全匹配。同时对附红细胞体、巴氏杆菌DNA样品进行扩增没有出现扩增条带。阳性犬全血DNA稀释1 000倍后,依然可以有效地检测出虫体DNA。结果表明,所建立的PCR检测方法,具有极高的敏感性和特异性,可以运用于临床检测巴贝西虫病。One pair of specific primers was designed according to the 18sRNA gene sequence of canine Babesia,and then a PCR assay was established for detecting canine Babesia.A fragment in length of 319 bp was amplified by PCR and the sequence homology to those from GenBank was 100%.No PCR products were amplified from purified DNA of Eperythrozoon or Pasteurella.This method can detect the genomic DNA of Canine Babesia in 10-3 diluted whole blood DNA of infected dogs.This indicates that the PCR method was precise,sensitive and specific.

关 键 词：PCR 犬 巴贝西虫 18sRNA基因 

分 类 号：S829.2[农业科学—畜牧学]