二醋吗啡依赖患者血细胞胎盘型谷胱甘肽S-转移酶mRNA的表达  

Expression of glutathione S-transferase-π mRNA in blood cells of diamorphine addicts

作　　者：陈高明[1] 杨征[2] 李春海[2] 訾薇娜 刘广贤[2] 郭庆玲[2] 

机构地区：[1]解放军九四医院,南昌330002 [2]军事医学科学院附属医院,北京100850

出　　处：《中国药理学与毒理学杂志》2000年第1期69-71,共3页Chinese Journal of Pharmacology and Toxicology

摘　　要：定量 RT- PCR检测 33例二醋吗啡依赖患者和 1 9例正常对照血细胞中的胎盘型谷胱甘肽 S-转移酶 ( GST-π)表达水平 ,同时用银染法检测患者 T细胞的核仁机化区 ( NOR)面积比 ( Ag- NOR) .结果表明二醋吗啡依赖患者血细胞中 GST- π表达水平明显高于正常对照 ,但与性别 ,吸毒时间和吸毒方式无关 .The expression level of glutathione S transferase π(GST π) in the blood cells of 33 diamorphine addicts and 19 normal volunteers was measured by quantitative PCR, at the same time the nucleolar organizer region(NOR) in T lymphocyte was stained by silver nitrate solution and the ratio of the silver stained area to the total region area(Ag NOR) was obtained. The expression rate and level of GST π mRNA were significantly higher in diamorphine addicts than in normal control. The expression level of GST π mRNA was negatively related to Ag NOR, but not to sex, dependence time and taking way of diamorpline.

关 键 词：谷胱甘肽 S-转移酶 二醋吗啡 药物依赖 

分 类 号：R968[医药卫生—药理学]