半乳糖寡糖在体内和体外对双歧杆菌生长的影响  

EFFECT OF GALACTOOLIGOSACCHARIDES ON GROWTH OF BIFIDOBACTERIA IN VIVO AND IN VITRO

作　　者：王筱兰[1] 魏东芝[2] 陈少欣[1] 俞俊棠[1] 

机构地区：[1]华东理工大学生物反应器国家重点实验室,上海200237 [2]华东理工大学生物化学研究所

出　　处：《中国微生态学杂志》2000年第1期15-16,21,共3页Chinese Journal of Microecology

摘　　要：目的:利用固定化产β-半乳糖苷酶的嗜热脂肪芽孢杆菌连续合成的产物。方法:经柱层析分离纯化得到了纯半乳糖三寡糖和半乳糖四寡糖,用于体外双歧杆菌培养和动物试验。结果:证实了半乳糖寡糖能高效、专一地促进双歧杆菌的生长繁殖。结论:半乳糖寡糖具有改善小鼠肠道内菌群分布,降低小鼠盲肠内pH值的生理功能。Objective:Galactooligosacchrides (GOS) were prepared from lactose by transgalactosylation by immobilized β-galactosidase. Methods:Trisaccharide and tetrasaccharide were purified by column chromatography. Results:It was verified by animial test and cultureing bifidobacteria in vitro that GOS had the physiological function of promoting growth of bifidobacteria efficiently and speciall. Conclusions:imocedure eliminates the need for preparation and purification of the plasmid.

关 键 词：半乳糖寡糠 双歧杆菌 动物试验 

分 类 号：Q532.3[生物学—生物化学] Q939.139