结晶紫-ZnS-BSA光度法测定蛋白质的含量  

Determination of Protein by Crystal Violet-ZnS-BSA Spectrophotometry

作　　者：胡卫平[1] 焦嫚[1] 董学芝[1] 王鑫[1] 

机构地区：[1]河南大学化学化工学院环境与分析科学研究所,河南开封475004

出　　处：《食品研究与开发》2012年第5期114-117,共4页Food Research and Development

基　　金：国家自然科学基金资助项目(No.20875022);省部共建河南大学科研项目

摘　　要：以乙酸锌为锌源,硫化钠为硫源,巯基乙酸为修饰剂水相合成了纳米粒子ZnS。方法基于ZnS能与结晶紫反应使结晶紫的峰型发生改变,吸光度降低,加入不同量的牛血清白蛋白(BSA)反应后,吸光度逐渐增大,建立结晶紫-ZnS-BSA分光光度法测定蛋白质的新方法。结果表明,体系的吸光度A与BSA浓度在0.010 0 mg/mL～4.40 mg/mL范围内呈良好的线性关系,线性方程为A=2.040 6+0.460 50C(mg/mL),相关系数R=0.996 2,检出限为0.009 15 mg/mL。该法用于肉松、酸奶等食品中蛋白质含量的测定,并与标准方法(考马斯亮蓝法)做对照,结果满意。ZnS nanoparticles that have special spectral properties were prepared with Zinc acetate and Sodium sulfide and mercapto acetic acid as modifier by hydrothermal synthesis method.Based on the absorption peaks changed of cristal violet after ZnS reacted with cristal violet,and the absorbance increased after added Bovine Serum Albumin（BSA） to the systen.A new method about determination of protein was established.The results have revealed that the absorbance（A） of system has a good linear relationship with the concentration of BSA in the range of 0.010 0 mg/mL-4.40 mg/mL,and the linear equation was A=2.040 6＋0.460 50C（mg/mL）,relation coefficient（R） was 0.996 2,the limit of detection was 0.009 15 mg/mL.The method have been used for determination of protein in milk and floss,and compared with the standard method（coomassie briliant blue method）,the results were satisfactory.

关 键 词：ZNS 牛血清白蛋白 结晶紫 分光光度法 

分 类 号：O657.3[理学—分析化学] O629.73[理学—化学]