十枣汤对腹水型荷瘤小鼠血管内皮生长因子的影响  

Vascular Endothelial Growth Factor(VEGF) in Ascites Model Mice's Serum and Ascites with with the Usage of Ten Jujube Decoction

作　　者：李航森[1] 肖曼丽[2] 

机构地区：[1]湖北省中医院肿瘤科,武汉430061 [2]湖北大学,武汉430062

出　　处：《实用中医内科杂志》2012年第5期5-6,10,共3页Journal of Practical Traditional Chinese Internal Medicine

基　　金：湖北省教育厅项目(D200616003)

摘　　要：[目的]观察十枣汤对腹水型荷瘤小鼠血管内皮生长因子(VEGF)的影响。[方法]使用随机平行对照方法,将6～7周龄健康昆明种小鼠90只随机分为正常对照组、荷瘤模型组、5-FU组、十枣汤大、中、小剂量组,每组15只。正常对照组不予处理,其余小鼠局部消毒后,腹腔内注射腹水型H22肝癌瘤细胞株0.2mL/只。次日起5-FU对照组腹腔内注射5-FU 25mL/kg,隔日1次,共5次。十枣汤组小鼠按27g/kg、81g/kg、160g/kg不同剂量等体积十枣汤灌胃,0.4mL/次,1次/d,连续10天。荷瘤模型空白对照组等容积生理盐水灌胃。各组末次给药24h后(腹腔传代第7日),称体重,脱颈椎处死,眼眶取血,抽吸乳白色浓稠腹水。检测血清VEGF、腹水VEGF。[结果]血清VEGF模型组、5-FU组、中药低剂量组、中药中剂量组、中药高剂量组较正常对照组血清VEGF均有不同程度增高(P<0.01);与模型组相比,均有显著性差异(P<0.05);与5-FU组相比,中药组均无显著性差异(P>0.05);其中中药低剂量组与中药高剂量组相比有显著性差异(P<0.05)。腹水VEGF 5-FU组、中药低剂量组、中药中剂量组、中药高剂量组较模型组腹水VEGF均有不同程度降低(P<0.05);与5-FU组相比,各中药低剂量组有显著性差异;中药中、高剂量无显著性差异(P>0.05);中药低剂量与中药中、高剂量均有显著性差异(P<0.05);中药中剂量与中药高剂量相比较无显著性差异(P>0.05)。[结论]十枣汤对腹水型荷瘤小鼠血清VEGF、腹水VEGF有与5-FU类似的抑制作用,并与剂量呈量效关系,这可能是十枣汤治疗恶性腹水,延长生存期、改善生存质量的作用机制。[ Objective ] To study how the level of vascular endothelial growth factor （VEGF） in ascites model mice＇s serum and ascites with Ehrlich ascites carcinoma Cell Strain changed with the usage of Ten Ju- jube Decoction. To explore the efficacy and mechanism of Ten Jujube Decoction related with malignant asci- tes. [Method] Randomized, parallel control method will be 6 to 7 weeks of age, healthy Kunming mice 90 were randomly divided into normal control group, tumor - bearing model group, 5 - FU group, Ten, Jujube Decoction large, medium and small dose group, n = 15only. Normal control group not treated, local disinfection of the remaining mice after intraperitoneal injection of ascites H22 hepatoma cell line O. 2mL / only. 5 - FU following the date of the abdominal cavity in the control group injected with 5 - FU, 25mL/kg every other day, a total of five times. 11 KM group mice 27g/kg, 81g/kg, 160g/kg different dose volume Ten, Jujube Decoction gavage 0.4mL / 1 / d for 10 consecutive days. Tumor - bearing model control group of equal vol- ume of normal saline. After the last administration of each group 24 hours （ abdominal pass on behalf of the first 7 days） , weighed, by cervical dislocation were sacrificed, eyes blood, aspiration of milky white thick ascites. Serum levels of VEGF, ascites VEGF. [Results] The serum VEGF model group, 5 - FU group, traditional Chinese medicine low dose group, dose group in the traditional Chinese medicine, Chinese medicine high dose group compared with normal control serum VEGF increased in varying degrees （ P 〈 0.01 ） ; Com- pared with model group, there were significant differences （ P 〈 0.05 ） ; compared with 5 - FU group, the Medicines there was no significant difference （ P 〉 0.05 ） ~ Chinese Medicine low dose group and traditional Chinese medicine high dose group compared to the significant difference （ P 〈 0.05 ）. Varying degrees of ascites of VEGF 5 - FU group, traditional Chinese medicine low dose group, dose group in the traditional Chinese

关 键 词：腹水型荷瘤小鼠血管内皮生长因子 十枣汤 伤寒论 血清VEGF 腹水VEGF 5-FU 腹水型H22肝癌瘤细胞株 量效关系 随机平行对照研究 

分 类 号：R273[医药卫生—中西医结合] R285.6[医药卫生—中医肿瘤科]