分子鉴定背瘤丽蚌中弯弓蚌螨的隐藏种  

Molecular Identification to a Cryptic Species of Unionicola arcuata from Lamprotula leai （Gray）

作　　者：郭维[1] 吴浩彬[1] 文春根[1] 

机构地区：[1]南昌大学生物科学系,南昌330031

出　　处：《蛛形学报》2012年第1期17-21,共5页Acta Arachnologica Sinica

基　　金：国家自然基金（30571434）;江西省教育厅科技项目（GJJ12024）和江西省自然基金（0630122）资助项目

摘　　要：Unionicola sp．与弯弓蚌螨Uarcuata在形态结构上非常相似，为了鉴别弯弓蚌螨Uarcuata和Unionicola sp．，本研究扩增出Unionicola sp．12S rRNA的部分基因片段，与基因数据库中5种寄生蚌螨序列进行比较分析。结果显示，Unionicola sp．与弯弓蚌螨uarcuata遗传差异为0．145，而分子系统树表明，Unionicola sp．与弯弓蚌螨uarcuata聚为一支，具较近的亲缘关系。我们推断Unionicola sp．是弯弓蚌螨Uarcuata的1个隐藏种。这可能是由于不同寄主蚌造成了宿主隔离，从而引起了蚌螨遗传基因的分化。The morphological characters of Unionicola sp. and U. arcuata were very similar. To identify Unionicola sp. from U. areuata, a portion of the mitochondrial 12S rRNA gene was amplified, cloned, and sequenced from Unionieola sp., comparative sequence analysis with other five unionicolid mites of GenBank. The results indicated that coefficients of genetic distance of Unionieola sp. and U. areuata were 0.145, and molecular tree showed that Unionieola sp. and U. areuata were the sister groups. These results suggest that Unionieola sp. is a cryptic species of U. areuata. It might be resulted in the host isolation for the different host clam, and genetic differentiation of unionicolid mites were influenced by the host species.

关 键 词：寄生蚌螨 弯弓蚌螨 12S RRNA基因 隐藏种 

分 类 号：Q78[生物学—分子生物学]