非生物胁迫对油菜脯氨酸积累与BnGG2基因表达的影响  

Influences of abiotic stress on proline accumulation and BnGG2 gene expression in dwarf mutant(Brassica napus L.)

作　　者：任彩霞[1] 张艺琼[1] 王茂林[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2012年第3期665-671,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：国家"863"(2009AA101105);四川省十一五油菜育种攻关项目(2006yzgg)

摘　　要：测定了非生物胁迫处理后甘蓝型油菜矮化突变体NDF-1及其野生型"3529"游离脯氨酸含量和G蛋白γ亚基基因BnGG2的表达变化.结果表明,从表型上看,突变体比野生型更耐胁迫.NDF-1和"3529"中脯氨酸含量随高温、高盐和干旱胁迫时间递增,但NDF-1中脯氨酸含量低于"3529".NDF-1和"3529"中BnGG2基因均可被高盐(200 mmol/L NaCl)和干旱(20%PEG 6000)诱导,而被高温(40℃)抑制,NDF-1比"3529"反应更迅速明显.低温(4℃)胁迫后脯氨酸和BnGG2表达趋势变化均不明显.以上结果说明NDF-1比"3529"更耐高温、高盐和干旱胁迫,矮化突变体可能有较强的抗逆性.而BnGG2与非生物逆境应答有着密切的关系,参与了甘蓝型油菜的抗逆响应路径.The experiments were carried out on the proline content of both a dwarf mutant of Brassica napus , named NDF-1,and wild type “3529”. The expression of BnGG2 under different abiotic stresses was analyzed at different time points. Results showed that the mutant was more resistant to stress than the wild type. The proline content increased after salt, drought and high temperature at different time points, but the proline content of the NDF-1 was lower than that of the “3529”. The BnGG2 gene expression wasupregulated in salt （200mM NaCl） and drought stress （20% PEG 6000）, but downregulated in heat （40 ℃）, and the expression of BnGG2 was increased rapidly and strongly in the NDF-1 than that in the “3529”. In the NDF-1 and the “3529”, proline content and BnGG2 gene expression pattern indicated no obvious changes after the low temperature （4 ℃） treatment. These results indicated that the NDF-1 was more resistant to salinity, drought and high temperature stress than the “3529”. The dwarf mutant probably has stronger resistance against abiotic stress. BnGG2 has close relationship with abiotic stress and might play a coordinating role in the pathway of stress resistance response in Brassica napus.

关 键 词：甘蓝型油菜 胁迫 脯氨酸 G蛋白γ亚基 

分 类 号：Q75[生物学—分子生物学]