细角螺卵子发生的细胞学和超微结构的研究  

Research on cytology and ultrastructure of oogenesis in Hemifusus ternatanus

作　　者：陆伟进[1] 蒋霞敏[1] 段雪梅[1] 姜小敏[1] 杜雪星[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《生态科学》2012年第2期173-181,共9页Ecological Science

基　　金：浙江省重大科技专项(2009C12076);浙江省大学生科技成果推广项目(013450932)资助

摘　　要：利用组织切片和透射电镜观察细角螺卵细胞发育的显微和超微结构,结果表明:细角螺卵原细胞期细胞核体积较大,呈椭圆形,核膜明显且有不规则的凹陷,细胞质内出现大量的线粒体和高尔基体。根据卵黄颗粒物的多少和大小可将卵母细胞分为前、中、后三个时期:前期卵母细胞细胞核内染色质浓缩,核仁可见,并出现核周间隙;中期卵母细胞内细胞核移向细胞的一端,核内染色质仍呈高电子密度状态,核仁不明显或消失;后期卵母细胞内的细胞核受挤压形状变得不规则,细胞质内可见少量的线粒体,大量的卵黄颗粒聚集在细胞质中并融合成很大的卵黄球。成熟期卵母细胞卵黄物质多且有较大的脂滴。Microstructttre and ultrastructure ofHemifusus termatamus egg development were observed using paraffin section and transmission electron microscopy. The results show that the nucleus ofH. termatamus oogonium is large. Oval and its nuclear membrane are irregular depression. A large number of mitochondria and Golgi bodies are in the cytoplasm. According to the amount and size of yolk particles, the oocytes can be divided into prophase, interim, and later phase. Chromatin condensates in the nucleus, nucleolus is visible and perinuclear space emerges in the preoocyte. The nucleus moves to one side of the cell in the interim oocyte and chromatin is in the high electron density state, but the nucleolus is not obvious or disappears. In the later oocytes the shape of nucleus is irregular because of extrusion. A small number ofmitochondria can be seen in the cytoplasm, but a large number of yolk granules fuse into a large ball in the cytoplasm. Yolk is rich and lipid droplet is big in the mature oocyte.

关 键 词：细角螺 卵子发生 细胞学 超微结构 

分 类 号：Q28[生物学—细胞生物学]