复方地西泮对蟾蜍鼻黏膜纤毛毒性作用的研究  

Study on Nasal Mucociliary Toxicity of Compound Diazepam In Toads

作　　者：王晓丹[1] 王晖[2] 吴江锋[3] 张瑞涛[3] 

机构地区：[1]三峡大学校医院,湖北宜昌443002 [2]广东药学院中药学院,广州510006 [3]三峡大学医学院,宜昌443002

出　　处：《中药新药与临床药理》2012年第3期255-258,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(81170412);湖北省教育厅自然科学研究项目(B20111203);三峡大学人才科研启动基金项目(KJ2009B076)

摘　　要：目的考察地西泮及复方地西泮(地西泮+薄荷醇)鼻腔给药对纤毛的毒性作用。方法以盐酸麻黄碱作阴性对照药,去氧胆酸钠作阳性对照药,考察地西泮、复方地西泮鼻腔给药对蟾蜍上颚纤毛持续运动时间(Persistent Vibration Duration,PVD)、纤毛持续运动百分率(Percentage of Persistent Vibration,PPV)、纤毛输送速率和蟾蜍上颚黏膜形态的影响。结果显微镜下观察,地西泮组、复方地西泮组对蟾蜍上颚纤毛形态无明显影响;地西泮组、复方地西泮组给药后PVD分别为(597.4±70.2)min、(606.2±55.4)min,与生理盐水组比较均无显著性差异(P>0.05);除1%盐酸麻黄碱组外,其余各组纤毛输送速率与生理盐水相比,差异有显著性(P<0.05)。结论地西泮、复方地西泮经鼻腔给药后,对蟾蜍鼻黏膜纤毛的毒性轻微,为鼻腔给药提供参考。Objective To study the nasal mucociliary toxicity of diazepam and compound diazepam（diazepam＋men- thol）. Methods Ephedrine hydrochloride was used as the negative control, and sodium deoxycholate was used as the positive control. In situ toad palate model was established as the experimental model to evaluate the effects of di- azepam and compound diazepam on persistent vibration duration（PVD） and percentage of persistent vibration（PPV） of toad palate cilia as well as the ciliary conveying velocity and palate mucosal features. Results Microscopic examina- tion of nasal mucociliary features of toad palate showed no significant difference in diazepam group, compound di- azepam group and physiologic saline group（P 〉 0.05）. The ciliary PVD in diazepam and compound diazepam group was （597.4±70.2） min and （606.2±55.4） rain respectively, showig no significant difference with physiologic saline group （P 〉 0.05）. Except for 1% ephedrine hydrochloride group, the difference of the ciliary conveying velocity was signif- icant between the medication groups and the saline group（P 〈 0.05）. Conclusion The diazepam and compound di- azepam show less mucociliary toxicity, which can be expected for intranasal administration.

关 键 词：地西泮 薄荷醇 鼻腔给药 纤毛毒性 

分 类 号：R285.5[医药卫生—中药学]