银杏黄酮苷元对兔颈动脉粥样硬化及NOX2、NOX4表达的影响  

Effect of Ginkgetin Flavone Aglycone on Carotid Atherosclerosis and Expression of NOX2 and NOX4 in Rabbits

作　　者：冯静[1] 何艳[2] 吴立荣[2] 刘兴德[2] 陈云[2] 李安敏[1] 

机构地区：[1]贵阳市第一人民医院,贵阳550002 [2]贵阳医学院附属医院心内科,贵阳550004

出　　处：《中药新药与临床药理》2012年第3期274-278,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：贵州省优秀科技教育人才省长专项资金项目(2005225)

摘　　要：目的探讨银杏黄酮苷元(GFA)对兔颈动脉粥样硬化及斑块处NOX2、NOX4表达的影响。方法 24只健康新西兰雄性大白兔随机分为对照组、假手术组、模型组、治疗组4组。对照组予普通饲料,其余各组均予高脂饲料,假手术组行颈外动脉结扎,模型组和治疗组行左颈总动脉内膜球囊损伤,治疗组每日予GFA(25mg·kg-1)干预4周,检测血脂水平;HE染色观察左颈总动脉形态,测定损伤处内膜面积及内膜中膜面积比值;免疫组化染色和实时荧光定量PCR(Real-time PCR)检测颈动脉斑块处NOX2、NOX4表达。结果与模型组比较,银杏黄酮苷元明显降低颈动脉内膜面积及内膜/中膜面积,下调斑块处NOX2、NOX4蛋白和mRNA表达(P<0.05)。结论银杏黄酮苷元可干预动脉粥样硬化病变形成,其作用可能与抑制NOX2、NOX4表达有关。Objective To investigate the effect of ginkgetin flavone aglycone （GFA） on carotid atherosclerosis （AS） and the expression of NOX2 and NOX4 in the rabbits. Methods Totally 24 healthy male New Zealand rabbits were ran- domly divided into 4 groups： normal control group, sham-operation group, model group（AS group）, GFA group. All rabbits were fed with high fat forage except that in the normal control group. Rabbits in the sham-operation group were given operation whithout endothelium injury, those in AS group and GFA group were given carotid balloon in- jury. GFA was administered 25 mg·kg^-1 daily to the treatment group by gavages. Serum lipids in 4 groups were detect- ed 4 weeks after operation. The carotid artery segments were harvested for histomorphological observation and for the determination of the area ratio of injuried carotid intima and media, and the expression of NOX2 and NOX4 was de tected through immunohistochemical staining and real-time fluorescence quantitative PCR（Real-time PCR）. Results GFA showed obvious effect on decreasing the area ratio of injuried carotid intima and media, and on downregulating NOX2 and NOX4 protein and mRNA expression （P 〈 0.05）. Conclusion GFA can inhibit atherosclerosis progress, which may be related to the inhibition of NOX2 and NOX4 expression.

关 键 词：银杏黄酮苷元 动脉粥样硬化 尼克酰胺腺嘌呤二核苷酸磷酸氧化酶 

分 类 号：R285.5[医药卫生—中药学]