兜唇石斛HPLC特征图谱研究  

Analysis of HPLC Characteristic Spectrum of Dendrobium Aphyllum

作　　者：杨丽娥[1] 黄月纯[2] 魏刚[1] 刘东辉[1] 石添香[1] 

机构地区：[1]广州中医药大学,广州510006 [2]广州中医药大学第一附属医院,广州510405

出　　处：《中药新药与临床药理》2012年第3期299-302,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省产学研结合项目(2009B090300333;2011B090400319);广州市番禺区科技计划项目(2010-专-12-11)

摘　　要：目的建立兜唇石斛HPLC特征图谱。方法采用Zorbax SB C18色谱柱;流动相为乙腈-0.1%甲酸溶液(梯度洗脱);检测波长为270 nm;柱温为35℃;流速为1.0 mL·min-1。结果兜唇石斛标示出42个共有峰;10批样品的相似度为0.901～0.961;兜唇石斛鲜品与兜唇石斛干品(水草枫斗)特征峰基本一致。结论此方法准确可靠,重复性好,为兜唇石斛的质量控制提供了方法依据。Objective To establish HPLC characteristic spectrum for Dendrobium aphyllum. Methods HPLC was performed on Zorbax SB Cls column, acetonitrile- 0.1% methanoic acid solution（gradient elution） was employed as a mobile phase, detection wavelength was 270 nm, column temperature was 35 ℃, and flow rate was 1.0 mL ·min^-1. Results Forty-two common peaks were separated in Dendrobium aphyllum, and the similarities of 10 batches were 0.901-0.961. Dried Dendrobium aphyllum and Fresh Dendrobium aphyllum had the similar specific peaks. Conclu- sion The method is accurate and reliable, has good reproducibility, and provides a reference for the quality control of Dendrobium aphyllum.

关 键 词：兜唇石斛 水草枫斗 兜唇石斛鲜品 特征图谱 HPLC 

分 类 号：R284.1[医药卫生—中药学]