狼毒质量标准改进研究  

Improvement of Quality Standard of Radix Euphorbiae Fischerianae

作　　者：严小红[1] 王灿坚[1] 毕福钧[1] 顾利红[1] 江英桥[1] 

机构地区：[1]广州市药品检验所,广州510160

出　　处：《中药新药与临床药理》2012年第3期321-324,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家科技支撑计划药品安全关键技术研究(2006BAI14B00)资助课题-常见与重要药品安全标准研究(2006BAI14B01);广州市食品药品监督管理局科技计划项目生狼毒特征图谱和岩大戟内酯B的药代动力学研究(2010GZFDA015)

摘　　要：目的研究和完善狼毒药材的质量标准。方法采用薄层色谱法对狼毒药材中的24-次甲基环木菠萝烷醇和岩大戟内酯B进行了鉴别;采用高效液相色谱(HPLC)法对狼毒乙素和岩大戟内酯B进行了含量测定。结果狼毒薄层鉴别专属性强,方法可行;狼毒乙素线性范围为0.013～0.863μg(r=0.9999),平均回收率为96.4%(RSD=1.4%,n=θ);岩大戟内酯B线性范围为0.007～0.496μg(r=0.9999),平均回收率为96.0%(RSD=2.1%,n=θ)。结论此方法准确、可靠,为狼毒药材的质量评价提供了依据。Objective To improve quality standard of Radix Euphorbiae Fischerianae. Methods The identification of 24-methylene cycloartanol and jolkinolide B in Radix Euphorbiae Fischerianae were carried out by TLC. The contents of 2,4-dihydroxy-6-methoxy-3 methylacetophenone and jolkinolide B were determinated by RP-HPLC. Results TLC identification of Radix Euphorbiae Fiseherianae was specific.The linear range of 2, 4-dihydroxy-6-methoxy- 3- methylacetophenone was 0.013-0.863 μg（r=0.9999）, and the mean recovery was 96.4 %（RSD=1.4 %, n=6）. The linear range of jolkinolide B was 0.007-0.496 Ixg（r=0.9999）, and the mean recovery was 96.0 %（RSD=2.1%, n= 6）. Conclusion This method is simple, effeetiveand precise. It can be used for the quality control of Radix Euphor- biae Fiseherianae.

关 键 词：狼毒 质量标准 狼毒乙素 岩大戟内酯B 

分 类 号：R284.1[医药卫生—中药学]