高效毛细管电泳法分析库拉索芦荟中的多种成分  

Multicomponent Analysis of Aloe Barbadensis Mill by High-performance Capillary Electrophoresis

作　　者：吴小芳[1] 刘丹[1] 钟佳胜[1] 王芝[1] 李婷[1] 万金志[1] 

机构地区：[1]中山大学药学院药物分析实验室,广州510006

出　　处：《中药新药与临床药理》2012年第3期331-335,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：2005年度广东省中医药管理局计划项目(1050163);2005年度珠海市科技计划项目(PC20051072)

摘　　要：目的建立分离芦荟苷同分异构体及同时测定不同库拉索芦荟样品中芦荟苷、异芦荟色苷D、芦荟大黄素含量的毛细管区带电泳法(CZE),并对各种影响电泳分离的因素进行了探讨。方法以芦丁为内标,采用未涂层弹性熔融石英毛细管柱(57 cm×75μm,有效长度50 cm),以20 mmol·L-1硼砂+2 mmol·L-1β-环糊精(pH=9.65)为背景电解质溶液,分离电压为18 kV,重力进样5 s(高度15 cm),检测波长365 nm。结果芦荟苷的一对同分异构体能够达到良好的分离,以芦丁为内标,库拉索芦荟样品中的芦荟苷、异芦荟色苷D、芦荟大黄素3种成分在7 min内得到良好的分离测定。结论此方法简便,快速,成本低,可用于芦荟样品的质量控制。Objective A capillary zone electrophoresis（CZE） method was established for the separation of aloin iso- mers and simultaneous determination of aloin, aloe emodin and isoaloeresin D in different samples of Aloe Barbaden- sis Mill, besides, the influential factors of CZE separation were discussed. Methods Rutin was used as the internal standard. The separation was performed on a uncoated fused silica capillary （57 cm × 75 μm, an effective length of 50 cm）, 20 mmol·L^-1 of borax and 2 mmol·L^-1 of β-CD buffer solution（pH = 9.65） was selected as the running buffer and the voltage was setting at 18 kV. The samples were injected by gravity（5 s, 15 cm）, and the detection wavelength was 365 nm. Results Aloin isomers can be well seperated. Aloin, aloe emodin and isoaloeresin D in dif- ferent samples of Aloe can be well-separated and be determined within 7 min with rutin as internal standard. Conclu- sion This method manifests properties as being simple, quick and economical. It is likely to be a better way for qual- ity control of Aloe Barbadensis Mill.

关 键 词：高效毛细管电泳法 库拉索芦荟 芦荟苷 异构体 异芦荟色苷D 芦荟大黄素 

分 类 号：R284.1[医药卫生—中药学]