中药复方消可宁附子总生物碱的醇提工艺  

The extraction process of active component of ′Xiaokening′—total alkaloid from Aconitum carmichaeli Debx

作　　者：郑燕影[1] 魏群利[1,2] 

机构地区：[1]南京军区南京总医院,江苏南京210002 [2]徐州医学院药学院临床药理教研室,江苏徐州221009

出　　处：《中国生化药物杂志》2012年第3期248-250,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：南京军区十一五医药卫生科研项目(08MA098);江苏省博士后基金项目(0901065C)

摘　　要：目的建立附子总生物碱的提取工艺。方法通过正交试验,以制附子总生物碱含量为指标,考察料液比,提取时间和提取温度等工艺参数对总生物碱提取效果的影响。结果优选出的最佳提取条件为:附子粉加10倍量的55%乙醇,浸泡1 h,70℃回流提取2次,每次1.0 h。结论该提取工艺操作简单,无有毒有机溶剂残留,具有良好的可操作性。Purpose To establish the extraction process of total alkaloid of ＇Xiaokening＇ from Aconiturn carmichaeli Debx. Methods The effects of extractants, solid liquid ratio, extraction time, extraction temperature, etc. were studied by the orthogonal design and the acquisition ration of total alkaloid acted as the examination standard. Results The optimum extraction condition were as follows： 10 folds of volumes of 55% medical grade ethanol,were immersed 1 h,water bath at 55 ℃for 1 and 2 times. Conclusion The extraction method is simple and practicable. The extracted alkaloids contain no poisonous organic solvents.

关 键 词：消可宁 制附子 总生物碱 提取工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学]