水解法提取纯化刺槐素的工艺研究  

Extraction and purification technique of acacetin by hydrosis

作　　者：朱性海[1] 王秀敏[1] 连惠龙[1] 金鑫[1] 李丽萍[1] 

机构地区：[1]厦门大学药学院,福建厦门361005

出　　处：《中国生化药物杂志》2012年第3期275-277,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：福建省科技计划重点项目(No.2011Y0051);厦门市科技计划项目(No.3502Z20103009)

摘　　要：目的优选野菊花中刺槐苷的水解工艺。方法采用均匀设计法,以高效液相色谱法(HPLC)测定野菊花中刺槐素含量,探讨影响刺槐素提取效率的因素,并筛选出最佳提取条件。结果最佳水解工艺为盐酸与药液比为5∶2,盐酸浓度3 mol/L,水解时间为3 h,水解温度为100℃。结论 HPLC法测定野菊花中刺槐素含量的方法,快速、可行。均匀设计法优选的野菊花中刺槐素提取工艺,操作简单,节约时间,提取率比原工艺有明显提高。Purpose To optimize the hydrosis technique of acaciin in Chrysanthemum. Methods With the amounts of acacetin as observation parameters by HPLC ,uniform design method was used for the optimization of extraction. Results The best hydrosis condition was as follows：The ratio of hydrochloric acid to liquid is 5：2,hydrochloric acid is 3 mol/L,time is 3 h,and temperature is 100 ℃. Conclusion The determination methods of acacetin content by HPLC is fast and feasible. The optimized condition is effi- cient, economic, and simple. The extraction rate is more significantly improved than the original process.

关 键 词：野菊花 刺槐素 均匀设计 水解工艺 

分 类 号：R284.2[医药卫生—中药学]