肺炎链球菌基因PBP1a变异:172 SSN→SSV(Asn172Val)  

Mutation of PBP1a from Streptococcus pneumoniae:172 SSN→SSV(Asn172Val)

作　　者：张春玲[1] 陈贤君[1] 蔡玲英 牛津[1] 李招云[1] 

机构地区：[1]浙江省台州市中心医院医学检验科,浙江台州318000 [2]浙江省温岭市人民医院医学检验科,浙江台州318000

出　　处：《中国卫生检验杂志》2012年第5期947-949,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省台州市科技局科技计划项目(091ky05)

摘　　要：目的:通过对台州地区β-内酰胺类抗生素耐药的肺炎链球菌青霉素结合蛋白基因的研究,分析肺炎链球菌的耐药机制。方法:收集2009年-2010年浙江台州地区63例肺炎链球菌临床株,进行培养、鉴定及药敏试验,并对耐药菌株行青霉素结合蛋白PBPs基因的PCR扩增和氨基酸序列分析。结果:研究结果显示PBP1a基因的变异位点除了以往报道的KTG保守序列之后变异为Thr574Ala,Ser575Thr,Gln57Gly,Phe577Tyr,和STMK保守区的Thr371Ala,本研究中发现172位氨基酸变异SSN→SSV。并且该菌株成高水平耐药。结论:新的氨基酸变异可能进一步降低了S.pneumoniae对β-内酰胺类抗生素的亲和力。Objective：To observe the Streptococcus pneumoniae resistant mechanism through the study on gene of Penicillin binding protein（PBPs）.Methods： 63 S.pneumoniae strains were collected from 2009 to 2010 in Taizhou area for culture,identification and susceptibility test.Then PBPs in drug-resistant strains were amplified by PCR and conducted DNA analysis.Results： The results showed that a novel PBP1a gene mutation site was detected： Asn172Val,in addition to the KTG（Thr574Ala,Ser575Thr,Gln57Gly,Phe577Tyr）and STMK（Thr371Ala） previously reported sequence variations.The mutation strains showed high resistance level to β-lactam antibiotics.Conclusion： Amino acids variation may further reduce the affinity of S.pneumoniae to the β-lactam antibiotics.

关 键 词：肺炎链球菌 Β-内酰胺类抗生素 PBP1a基因 

分 类 号：R378.12[医药卫生—病原生物学]