环鸟苷酸信使系统参与rbcS基因表达调控初报  

The Involvement of cGMP Signaling Pathway in the Repression of rbcS Gene

作　　者：张素巧[1] 乔红[1] 马力耕[1] 孙大业[1] 

机构地区：[1]河北师范大学生物系,河北石家庄050016

出　　处：《河北师范大学学报（自然科学版）》2000年第1期102-104,共3页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金!重点项目 ( 3 973 0 2 3 0 );国家自然科学基金资助项目!( 3 9870 3 65 )

摘　　要：以转 rbc S-GUS基因烟草悬浮细胞为材料 ,通过用荧光光度法检测报告基因 GUS酶的活性的方法 ,发现暗中培养的转基因烟草悬浮细胞经细胞外 Ca M或红光处理后 ,rbc S基因表达明显增加 (分别增加 2～ 3倍和 7倍 ) ,而细胞外 Ca M和红光同时处理却只使 rbc S基因表达增加 4～ 5倍 .在暗中培养的转基因烟草悬浮细胞介质中加入 8-Br-c GMP使 rbc S基因表达降低 50 %左右 ,而加入鸟苷酸环化酶拮抗剂 LY83 583和 Genestin则使 rbc S基因表达增加约 50 % .上述结果表明 ,细胞外 Ca M和红光在调控 rbc S基因表达时存在拮抗关系 ,并且 c GMP信使系统可能参与了 rbcWe used transgenic tobacco with rbcS GUS fusion gene as experimental materials and examined the expression of rbcS GUS gene through the activity analysis of GUS.Adding purified CaM to suspension cultured tobacco cells resulted in an increase of the expression of rbcS about 2~3 folds higher than that of control and red light illumination resulted in 7 folds higher.But purified CaM together with red light treatment only increased the expression of rbcS about 4~5 folds.The expression of rbcS decreased 50% after adding 8 Br cGMP to the suspension cultured cells in dark,while adding LY83583 and Genestin increased the expression of rbcS about 50%. These data showed extracellular CaM and red light signaling pathways may inhibit each other,and cGMP signaling pathway may involve in the repression of rbcS gene.

关 键 词：转基因烟草 rbcS基因表达 环鸟苷酸信使系统 

分 类 号：S572.034[农业科学—烟草工业] Q78[农业科学—作物学]